Volume 3 Supplement 1
Preliminary analysis of the sinusoidal endothelium and space of Disse in ageing Papio hamadrayas
© Cogger et al; licensee BioMed Central Ltd 2004
Published: 14 January 2004
In the past it has been reported that the liver is free of any major age-related morphological changes apart from binucleate hepatocytes and lipofuscin deposition. Diminished hepatic blood flow and mass have been considered to be responsible for the decreased metabolic capacity seen in old age . Recently we studied the ageing rat liver  and the ageing human liver  and found that there are a number of age-related changes to the perisinusoidal region at the ultrastructural level.
The animals used in this study were from a captive breeding colony of Papio hamadrayas. The experimental protocol was approved by the Central Sydney Area Health Service animal welfare committee. Liver tissue was collected by needle biopsy or from fresh post mortem animals. Half of each specimen was fixed for light microscopy and immunohistochemistry in 4% buffered paraformaldehyde and the other half was fixed for electron microscopy with 2% gluteraldehyde/ 3% paraformaldehyde in 0.1 M sodium-cacodylate buffer (0.1 M Sucrose, 2 mM CaCl2). Specimens fixed for light microscopy and immunohistochemistry were embedded in paraffin blocks. Sections from each animal were stained with H & E, Masson's trichrome and Wilder's reticulin. Immunohistochemistry was also used to detect the expression of Collagen IV, Laminin, Synaptophysin, Fibronectin, von Willebrands factor (vWF) and CD68. Tissue for transmission and scanning electron microscopy was prepared by standard methods  and randomly sampled for examination. Transmission electron micrographs were taken at 17 000– magnification (Philips CM120) and scanning electron micrographs were taken at 40 000– magnification (Philips XL30).
The findings confirm that significant age-related changes occur in the perisinusoidal region of the baboon liver. These changes are similar to those we have previously reported in rats and humans which suggests that pseudocapillarization is a widespread ageing change. Defenestration of the endothelium is associated with increased plasma lipids and may explain the association of old age with atherosclerosis , an other diseases potentially linked to impaired hepatic function.
- Le Couteur DG, McLean AJ: The aging liver. Drug clearance and an oxygen diffusion barrier hypothesis. Clinical Pharmacokinetics. 1998, 34: 359-373. 10.2165/00003088-199834050-00003.View ArticlePubMedGoogle Scholar
- Le Couteur DG, Cogger VC, Markus AM, Harvey PJ, Yin ZL, Ansselin AD, McLean AJ: Pseudocapillarization and associated energy limitation in the aged rat liver. Hepatology. 2001, 33: 537-543. 10.1053/jhep.2001.22754.View ArticlePubMedGoogle Scholar
- McLean AJ, Cogger VC, Chong GC, Warren A, Markus AM, Dahlstrom JE, Le Couteur DG: Age-related pseudocapillarization of the human liver. Journal of Pathology. 2003, 200: 112-117. 10.1002/path.1328.View ArticlePubMedGoogle Scholar
- Wisse E, Wilde AD, Zanger RD: Perfusion fixation of human and rat liver tissue for light and electron microscopy: a review and assessment of existing methods with special emphasis on sinusoidal cells and microcirculation. In: The science of biological specimen preparation for microscopy and microanalysis. Edited by: Ravel JP, Barnard T, Haggis GH. 1984, Chicago, Scanning Electron Microscopy Inc, 31-38.Google Scholar
- Le Couteur DG, Fraser R, Cogger VC, McLean AJ: Hepatic pseudocapillarisation and atherosclerosis in ageing. Lancet. 2002, 359: 1612-1615. 10.1016/S0140-6736(02)08524-0.View ArticlePubMedGoogle Scholar
This article is published under license to BioMed Central Ltd. This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.