- Open Access
Intercellular Adhesive Structures Between Stellate Cells – An Analysis in Cultured Human Hepatic Stellate Cells
© Imai et al; licensee BioMed Central Ltd 2004
- Published: 14 January 2004
To investigate whether or not hepatic stellate cells can form intercellular junctions with each other, we cultured human stellate cells (LI90) on different kinds of substrata. Intercellular junctions were detected between these cultured stellate cells by transmission electron microscopy (TEM). The molecular components of the intercellular adhesive structures were identified by immunofluorescence microscopy. Immunofluorescence for cadherin and catenins was detected at the adhesion sites between the cultured stellate cells. Thus, the intercellular junctions were indicated to be adherens junctions at the molecular level. The junctions developed in the cultured stellate cells irrespective of the type of substratum. These data suggest that the junctional formation between the stellate cells occurs in vivo as well as in vitro.
- Stellate Cell
- Hepatic Stellate Cell
- Immunofluorescence Microscopy
- Adherens Junction
- Molecular Component
In the normal liver, hepatic stellate cells are located between sinusoidal endothelial cells and hepatic parenchymal cells. The stellate cells and their processes adhere to the endothelial cells on one hand and the other hand contact with the parenchymal cells . However, the presence of intercellular junctions between stellate cells has not yet been fully established. The processes of stellate cells coexist with the three-dimensional extracellular matrix (ECM) within the space [2, 3]. The three-dimensional ECM components and the long numerous processes of stellate cells make a complicated structure. Hence, it is difficult to elucidate cell-cell junctions between hepatic stellate cells in vivo. To investigate whether or not hepatic stellate cells can form intercellular junctions with each other, we cultured stellate cells, and then morphologically examined the structure of the cultured cells with special reference to intercellular junctions.
For morphological examinations by electron microscopy, we cultured LI90 cells on the different types of substrata, namely, non-coated polystyrene culture dishes, type I collagen gel, or Matrigel. The structures of cultured LI90 cells were examined with special reference to intercellular adhesive structures in the cultured cells by TEM. For immunofluorescence microscopy, we seeded LI90 cells into glass-bottom dishes. The molecular components of the intercellular adhesive structures formed by the cultured cells were identified by indirect immunofluorescence microscopy for pan-cadherin, alpha- and beta-catenins. Immunostained cells were examined with a confocal laser-scanning microscope (LSM).
Cultured LI90 cells formed intercellular adhesive structures with each other. At the sites of intercellular junctions, plasma membranes of the cells were arranged in parallel and characterized by electron-dense cytoplasmic plaques. The adhesive structures were morphologically identified as adherens junctions at the fine structural level. As cadherin family, alpha- and beta-catenins are molecular components of adherens junctions , the intercellular adhesive structures formed by the cultured LI90 cells were presumed to be adherens junctions. Stellate cells coexist with many kinds of extracellular matrix components within the normal perisinusoidal space [1–3]. The junctions developed in the cultured stellate cells irrespective of the type of culture substratum. These data suggest that the junctional formation between stellate cells occurs in vivo as well as in vitro. Thus, the hepatic stellate cells may participate in the structural organization of the cells in liver lobules through the formation of intercellular junctions between themselves.
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