Confocal laser scanning microscopy of M2-Pk and biomarkers of sinusoidal liver cells. Double immunofluorescence of M2-Pk (green, A', B', C') with F4/80 (red, A), with GFAP (red, B) and with vimentin (red, C). Merged images are shown in A'', B'' and C'', respectively. Colocalization of GFAP (red, D, E) with vimentin in a pericentral (green, D') and in a periportal (green, E') region is shown in D'' and E'', respectively. Faint red fluorescence of the membranes of biliary cells is indicated by the white arrow in E. Colocalization of CD31 immunoreactivity (red, F) with vimentin (green, F') is shown in F''. Immunofluorescence stainings were recorded by Confocal Laser Scanning microscopy. Bar = 20 μm.