Screening for inhibitors of trans-differentiation in rat and human HSCs – Part 1. Rat HSCs were isolated and cultured for 2 days (T0) whereupon cells were treated with the indicated compound as outlined in methods section. After 9 days, cells were analyzed by Western blotting for α-smooth muscle actin (α-sma). Each lane contains 10 μg total protein/lane, results typical of at least 3 separate experiments (a). Human HSCs were treated with the indicated compound and confluence determined in randomly selected fields. Data are the mean and standard deviation confluence at day 12 of 3 separate treatment dishes from the same donor, typical of at least 3 separate donors (b).