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Table 7 Volumetric assessment of α-napthylisothiocyanate (ANIT) exposed livers

From: Non invasive in vivo investigation of hepatobiliary structure and function in STII medaka (Oryzias latipes): methodology and applications

30 dpf 2.5 μM ANIT, 48 hpd Volume μm3 % Volume SA μm2
Intrahepatic Biliary Passageways 11743 0.5% 37818
Vasculature 159187 7.3% 92092
Parenchyma 2023674 92.7% 0
Hepatocellular 2011931 92.2% 0
Liver Corpus 2182861 - 156422
Stack size: 230 × 230 × 89 μm, 1 μm sections, scaling/voxel size 0.45 × 045 × 1 μm, 3D volume analysis ~30% total liver volume
30 dpf 1 μm ANIT, 48 hpd Volume μm 3 % Volume SA μm 2
Intrahepatic Biliary Passageways 100354 1.18% 164119
Vasculature 421852 7.92% 207939
Parenchyma 8083460 92.08%  
Hepatocellular 7983106.00 100.00%  
Liver Corpus 8505312   546491
Stack size: 325 × 325 × 89 μm, 1 μm section, scaling/voxel size 0.64 × 0.64 × 1 μm. ~30% total liver volume
  Mean: 8 – 30 dpf 2.5 μM ANIT 30 dpf 1 μM ANIT 30 dpf
CTRL vs. Treated % Volume % Volume % Volume
Intrahepatic Biliary Passageways 0.97% 0.5% 1.18%
Vasculature 7.51% 7.3% 7.92%
Parenchyma 92.49% 92.7% 92.08%
Hepatocellular 91.52% 92.2% 92.15%
Liver Corpus    
  1. Volumetric comparisons between normal and ANIT exposed livers. Indices are summarized in the lower part of Table 7 (see also Table 5). Medaka treated with 1 μM ANIT and analyzed (3D reconstructions, volumetrics) at 30 days exhibited an increase in volume of intrahepatic biliary passageways. Medaka treated with 2.5 μM ANIT and analyzed at 30 days exhibited a decrease in intrahepatic biliary volume. Where biliary volumes were changed in ANIT exposed animals, other indices of vasculature, parenchymal and hepatocellular volume remained relatively well conserved across normal and treated medaka. These results, while from only 2 treated animals, may suggest a choleretic response at low ANIT exposure concentrations, and cholestatic like response at higher aqueous ANIT concentrations. Due to the time consuming nature of 3D reconstructions, only 2 livers of treated animals were reconstructed in full.