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Table 5 Hepatobiliary volumetrics from in vivo based 3D investigations.

From: Non invasive in vivo investigation of hepatobiliary structure and function in STII medaka (Oryzias latipes): methodology and applications

8 dpf Volume μm3 % Volume SA μm2
Intrahepatic Biliary Passageways 3653 1.03% 12259
Vasculature 22416 6.33% 23586
Parenchyma 331569 93.67%  
Hepatocellular 327916 92.64%  
Liver Corpus 353985 - 47310
Stack size: 115 × 115 × 50 μm, sections: 1 μm, scaling/voxel size: 0.2 × 0.2 × 1 μm, 3D volume analysis: ~50% total liver volume
12 dpf Volume μm 3 % Volume SA μm 2
Intrahepatic Biliary Passageways 19877 0.86% 47808
Vasculature 199157 8.60% 127215
Parenchyma 2117236 91.40%  
Hepatocellular 2097359 90.54%  
Liver Corpus 2316393 - 318879
Stack size: 230 × 230 × 90 μm, sections: 1 μm, scaling/voxel size: 0.45 × 0.45 × 1 μm, 3D volume analysis: ~50% total liver volume
30 dpf Volume μm 3 % Volume SA μm 2
Intrahepatic Biliary Passageways 11435 1.01% 24871
Vasculature 85837 7.60% 38682
Parenchyma 1043352 92.40%  
Hepatocellular 1031917 91.39%  
Liver Corpus 1129189 - 272427
Stack size: 325 × 325 × 178 μm, sections: 1 μm, scaling/voxel size: 0.64 × 0.64 × 2 μm, 3D volume analysis: ~15% total liver volume
  1. Hepatobiliary metrics obtained from 3D reconstructions of the hepatobiliary system at 8, 12 and 30 dpf. A consistent relationship among the volume of intrahepatic biliary passageways, vasculature, hepatocellular volume, and parenchymal volume, in relation to total liver volume analyzed (liver corpus), was found across each stage of development. Given are the dimensions of in vivo confocal stacks from which 3D reconstructions were made, as well as voxel size (a proxy for resolution). Also given are estimates for the total volume of liver analyzed; for instance, at 8 dpf, it is estimated that the stack size was ~50% of total liver volume, and at 30 dpf, the stack dimensions are estimated to comprise ~15% of total liver volume. Total volume of each 3D reconstruction corresponds to volume of liver corpus. Parenchymal volume is comprised of hepatocellular space, BPDECs and intrahepatic biliary passageways. SA = Surface Area; Vol = Volume