Non invasive in vivo investigation of hepatobiliary structure and function in STII medaka (Oryzias latipes): methodology and applications

Table 5 Hepatobiliary volumetrics from in vivo based 3D investigations.

8 dpf	Volume μm³	% Volume	SA μm²
Intrahepatic Biliary Passageways	3653	1.03%	12259
Vasculature	22416	6.33%	23586
Parenchyma	331569	93.67%
Hepatocellular	327916	92.64%
Liver Corpus	353985	-	47310
Stack size: 115 × 115 × 50 μm, sections: 1 μm, scaling/voxel size: 0.2 × 0.2 × 1 μm, 3D volume analysis: ~50% total liver volume
12 dpf	Volume μm ³	% Volume	SA μm ²
Intrahepatic Biliary Passageways	19877	0.86%	47808
Vasculature	199157	8.60%	127215
Parenchyma	2117236	91.40%
Hepatocellular	2097359	90.54%
Liver Corpus	2316393	-	318879
Stack size: 230 × 230 × 90 μm, sections: 1 μm, scaling/voxel size: 0.45 × 0.45 × 1 μm, 3D volume analysis: ~50% total liver volume
30 dpf	Volume μm ³	% Volume	SA μm ²
Intrahepatic Biliary Passageways	11435	1.01%	24871
Vasculature	85837	7.60%	38682
Parenchyma	1043352	92.40%
Hepatocellular	1031917	91.39%
Liver Corpus	1129189	-	272427
Stack size: 325 × 325 × 178 μm, sections: 1 μm, scaling/voxel size: 0.64 × 0.64 × 2 μm, 3D volume analysis: ~15% total liver volume

Hepatobiliary metrics obtained from 3D reconstructions of the hepatobiliary system at 8, 12 and 30 dpf. A consistent relationship among the volume of intrahepatic biliary passageways, vasculature, hepatocellular volume, and parenchymal volume, in relation to total liver volume analyzed (liver corpus), was found across each stage of development. Given are the dimensions of in vivo confocal stacks from which 3D reconstructions were made, as well as voxel size (a proxy for resolution). Also given are estimates for the total volume of liver analyzed; for instance, at 8 dpf, it is estimated that the stack size was ~50% of total liver volume, and at 30 dpf, the stack dimensions are estimated to comprise ~15% of total liver volume. Total volume of each 3D reconstruction corresponds to volume of liver corpus. Parenchymal volume is comprised of hepatocellular space, BPDECs and intrahepatic biliary passageways. SA = Surface Area; Vol = Volume