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Table 4 Hepatobiliary morphometrics from in vivo based 3D investigations.

From: Non invasive in vivo investigation of hepatobiliary structure and function in STII medaka (Oryzias latipes): methodology and applications

Biliary   Mean Mode SD Min Max   n =
8 dpf IHBP Segment Length (μm) 11.8 11.6 1.2 10.0 17.0   68.0
12 dpf   11.9 11.6 1.4 9.2 15.8   39.0
30 dpf   12.8 11.3 1.6 9.8 16.5   34.0
40 dpf   11.6 11.1 1.3 8.5 14.6   42.0
8 dpf Degree 120 119.8 118.0 6.7 100.0 137.0   81.0
12 dpf   119.8 127.0 7.2 103.0 136.0   61.0
30 dpf   117.2 123.0 20.8 11.5 134.0   33.0
40 dpf   124.4 123.0 8.5 103.0 146.0   73.0
8 dpf Degree 90 91.4 91.0 4.2 83.0 99.1   15.0
12 dpf   89.9 89.5 5.1 80.6 98.8   16.0
30 dpf   91.0 - 8.0 78.0 101.0   6.0
40 dpf   98.5 103.0 7.9 84.4 109.0   12.0
8 dpf Degrees Other        
12 dpf   56.6 50.0 14.7 44.0 88.0   15.0
30 dpf   68.7 40.0 37.6 40.0 163.0   10.0
40 dpf   66.4 49.0 32.6 41.0 154.0   9.0
8 dpf IHBP Diameter (μm) 1.3 1.3 0.3 0.9 1.8   30.0
12 dpf   1.4 1.4 0.2 1.0 2.0   37.0
30 dpf   1.3 1.2 0.3 0.9 2.2   22.0
40 dpf   1.3 1.3 0.5 0.5 2.6   23.0
8 dpf BPD Segment Length (μm)        
12 dpf   6.2 6.3 1.5 3.8 9.4   15.0
30 dpf   7.4 6.2 2.6 3.9 13.5   15.0
40 dpf   8.3 - 2.5 4.3 15.6   21.0
Cellular
8 – 40 dpf Hepatocyte Diameter (μm) 11.3 11.8 1.0 8.4 13.5   48.0
8 – 40 dpf BPDEC (μm) 5.5 4.3 1.3 3.3 10.2   48.0
8 dpf Muralium/Tubule Diameter
(height variance of 20 μm to > 100 μm)
21.2 22.7 3.6 13.5 32.7   36.0
20 dpf   29.6 34.5 8.6 19.8 63.4   38.0
30 dpf   26.0 26.9 5.7 17.3 43.8   32.0
40 dpf   24.8 24.5 6.3 16.9 48.7   36.0
Vasculature
8 dpf Sinusoid Diameter (height variance of 8 μm to 31 μm) 6.9 6.7 0.9 5.2 8.8   26.0
20 dpf   7.5 6.9 1.3 5.4 11.3   32.0
30 dpf   7.6 7.6 1.6 4.9 12.1   32.0
40 dpf   7.9 7.1 1.9 3.3 12.6   34.0
20 dpf Hepatic Vein Diameter (μm) 18.9       
30 dpf   18.3       
20 dpf Portal Vein Diameter (μm) 13.9       
30 dpf   14.7       
  1. Metrics obtained from 3D reconstructions of the hepatobiliary system at 8, 12, 20, 30 and 40 dpf provided highly accurate morphological assessment of components of the hepatobiliary system. IHBP segment length corresponds to average length of the canaliculus, which was found to be approximately the same as hepatocyte diameter. Bile preductule (BPD) segment length is length of bile passageway surrounding BPDECs, a length that approximates BPDEC diameter. In vivo indices from 3D analyses correlated will with ex vivo ultrastructural (TEM) indices, and in vivo 2D metrics.