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Table 4 Hepatobiliary morphometrics from in vivo based 3D investigations.

From: Non invasive in vivo investigation of hepatobiliary structure and function in STII medaka (Oryzias latipes): methodology and applications

Biliary

 

Mean

Mode

SD

Min

Max

 

n =

8 dpf

IHBP Segment Length (μm)

11.8

11.6

1.2

10.0

17.0

 

68.0

12 dpf

 

11.9

11.6

1.4

9.2

15.8

 

39.0

30 dpf

 

12.8

11.3

1.6

9.8

16.5

 

34.0

40 dpf

 

11.6

11.1

1.3

8.5

14.6

 

42.0

8 dpf

Degree 120

119.8

118.0

6.7

100.0

137.0

 

81.0

12 dpf

 

119.8

127.0

7.2

103.0

136.0

 

61.0

30 dpf

 

117.2

123.0

20.8

11.5

134.0

 

33.0

40 dpf

 

124.4

123.0

8.5

103.0

146.0

 

73.0

8 dpf

Degree 90

91.4

91.0

4.2

83.0

99.1

 

15.0

12 dpf

 

89.9

89.5

5.1

80.6

98.8

 

16.0

30 dpf

 

91.0

-

8.0

78.0

101.0

 

6.0

40 dpf

 

98.5

103.0

7.9

84.4

109.0

 

12.0

8 dpf

Degrees Other

       

12 dpf

 

56.6

50.0

14.7

44.0

88.0

 

15.0

30 dpf

 

68.7

40.0

37.6

40.0

163.0

 

10.0

40 dpf

 

66.4

49.0

32.6

41.0

154.0

 

9.0

8 dpf

IHBP Diameter (μm)

1.3

1.3

0.3

0.9

1.8

 

30.0

12 dpf

 

1.4

1.4

0.2

1.0

2.0

 

37.0

30 dpf

 

1.3

1.2

0.3

0.9

2.2

 

22.0

40 dpf

 

1.3

1.3

0.5

0.5

2.6

 

23.0

8 dpf

BPD Segment Length (μm)

       

12 dpf

 

6.2

6.3

1.5

3.8

9.4

 

15.0

30 dpf

 

7.4

6.2

2.6

3.9

13.5

 

15.0

40 dpf

 

8.3

-

2.5

4.3

15.6

 

21.0

Cellular

8 – 40 dpf

Hepatocyte Diameter (μm)

11.3

11.8

1.0

8.4

13.5

 

48.0

8 – 40 dpf

BPDEC (μm)

5.5

4.3

1.3

3.3

10.2

 

48.0

8 dpf

Muralium/Tubule Diameter

(height variance of 20 μm to > 100 μm)

21.2

22.7

3.6

13.5

32.7

 

36.0

20 dpf

 

29.6

34.5

8.6

19.8

63.4

 

38.0

30 dpf

 

26.0

26.9

5.7

17.3

43.8

 

32.0

40 dpf

 

24.8

24.5

6.3

16.9

48.7

 

36.0

Vasculature

8 dpf

Sinusoid Diameter (height variance of 8 μm to 31 μm)

6.9

6.7

0.9

5.2

8.8

 

26.0

20 dpf

 

7.5

6.9

1.3

5.4

11.3

 

32.0

30 dpf

 

7.6

7.6

1.6

4.9

12.1

 

32.0

40 dpf

 

7.9

7.1

1.9

3.3

12.6

 

34.0

20 dpf

Hepatic Vein Diameter (μm)

18.9

      

30 dpf

 

18.3

      

20 dpf

Portal Vein Diameter (μm)

13.9

      

30 dpf

 

14.7

      
  1. Metrics obtained from 3D reconstructions of the hepatobiliary system at 8, 12, 20, 30 and 40 dpf provided highly accurate morphological assessment of components of the hepatobiliary system. IHBP segment length corresponds to average length of the canaliculus, which was found to be approximately the same as hepatocyte diameter. Bile preductule (BPD) segment length is length of bile passageway surrounding BPDECs, a length that approximates BPDEC diameter. In vivo indices from 3D analyses correlated will with ex vivo ultrastructural (TEM) indices, and in vivo 2D metrics.
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Comparative Hepatology

ISSN: 1476-5926