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Table 2 Endogenous fluorophores.

From: Non invasive in vivo investigation of hepatobiliary structure and function in STII medaka (Oryzias latipes): methodology and applications

 

Ex (nm)

Em (nm)

Flavoproteins: flavin adenine dinucleotide (FAD), flavin mononucleotide (FMN)

450

550

Pyridine Nucleotides: nicotinamide dinucleotide (NAD), nicotinamide dinucleotide phosphate (NADP), the reduced states fluoresce – NADPH, NADH

336

450

Tryptophan

295

329

Hydroxykynurenine glucoside (3-HGK)

520

550

Serotonin (5-HT)

290/366

340-440/510

5 HIAA

300

350

α-napthol

340

460

Bilirubin

435

500

Protoporphyrin

424/408

594/630

Collagen I

340/500

410/520

Elastin

330

405

Lipofuscin

300

420

Vitamin A

345

490

Vitamin E

295

335

Thiamin (B1)

366

430

Riboflavin (B2)

440

514

Salicylic Acid

319

408

  1. Given are some of the known endogenous fluorophores and their excitation (Ex) and emission (Em) wavelength(s).