Quantitative analysis of blood to bile transport in vivo (STII medaka, 12 dpf): β-Bodipy C5 Phosphocholine (HPC), Fluorescein Isothiocyanate (FTIC) and Bodipy C5 ceramide. Box plots (area averages) and statistical indices: Quantitative analysis of differential blood to bile transport between β-Bodipy C5 phosphocholine (HPC), fluorescein isothiocyanate (FTIC) and Bodipy C5 ceramide. Fluorophores were imaged in vivo at peak uptake times (60 minutes for C5 ceramide, 45 minutes for both FITC and HPC). Differences in blood to bile transport between all three fluorophores were suggested when measured values (means) of fluorescence intensity across sinusoid, cytosol and canalicular spaces were evaluated. The most marked differences were between β-Bodipy C5 phosphocholine and fluorescein isothiocyanate, and Bodipy C5 ceramide. For instance: Where ceramide and FITC showed no statistical difference in concentration in the canaliculus, there were marked differences in concentration, and temporal variation, in cytosol and sinusoid, suggesting differences in transport kinetics.