Production of inflammatory cytokines by LSECs. Secretion of IL-1β (a), IL-6 (b), or IL-10 (c) was measured in LSEC culture supernatants by ELISA at different time-points. Conditioned media were collected from LSECs cultured at hyperoxia (open bars) or normoxia (filled bars) at 24 hours intervals. Final concentrations were estimated from individual standard curves. Values are means of triplicate measurements. The results are representative data obtained from three independent experiments. Statistical analyses by t-student test: *P < 0.001.