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Table 2 Primary Antibodies used in Western blot experiments

From: Major HGF-mediated regenerative pathways are similarly affected in human and canine cirrhosis

Antibody Product size (kDa) Dilution Supplier
Goat anti-human HGF 80 1:1,000 Santa Cruz
Goat anti-human c-MET 145 1:1,000 Santa Cruz
Rabbit anti-human c-MET (Tyr1230/1234/1235) 169 1:1,000 Abcam
Rabbit anti-human/dog STAT3 86 1:2,500 BD Biosciences
Rabbit anti-human phospho-STAT3 (Ser727) 86 1:1,000 Cell Signalling
Mouse anti-human phospho-STAT3 (Tyr705) 86 1:1,000 Cell Signalling
Mouse anti-human/dog PKB 60 1:250 BD Biosciences
Rabbit anti-human phospho-PKB (Thr308) 60 1:1,000 Cell Signalling
Rabbit anti-human Erk1/2 42/44 1:1,000 Cell Signalling
Rabbit anti-human phospho-ERK1/2 (Thr202/Tyr204) 42/44 1:1,500 Cell Signalling
Rabbit anti-human p38-MAPK 38 1:500 Abcam
Rabbit anti-human phospho-p38-MAPK (Thr180/Tyr182) 38 1:1,000 Abcam
Mouse anti-human/dog Beta-actin (pan Ab-5) 42 1:2,000 Neomarkers