Table 2 Primary Antibodies used in Western blot experiments
From: Major HGF-mediated regenerative pathways are similarly affected in human and canine cirrhosis
Antibody | Product size (kDa) | Dilution | Supplier |
|---|---|---|---|
Goat anti-human HGF | 80 | 1:1,000 | Santa Cruz |
Goat anti-human c-MET | 145 | 1:1,000 | Santa Cruz |
Rabbit anti-human c-MET (Tyr1230/1234/1235) | 169 | 1:1,000 | Abcam |
Rabbit anti-human/dog STAT3 | 86 | 1:2,500 | BD Biosciences |
Rabbit anti-human phospho-STAT3 (Ser727) | 86 | 1:1,000 | Cell Signalling |
Mouse anti-human phospho-STAT3 (Tyr705) | 86 | 1:1,000 | Cell Signalling |
Mouse anti-human/dog PKB | 60 | 1:250 | BD Biosciences |
Rabbit anti-human phospho-PKB (Thr308) | 60 | 1:1,000 | Cell Signalling |
Rabbit anti-human Erk1/2 | 42/44 | 1:1,000 | Cell Signalling |
Rabbit anti-human phospho-ERK1/2 (Thr202/Tyr204) | 42/44 | 1:1,500 | Cell Signalling |
Rabbit anti-human p38-MAPK | 38 | 1:500 | Abcam |
Rabbit anti-human phospho-p38-MAPK (Thr180/Tyr182) | 38 | 1:1,000 | Abcam |
Mouse anti-human/dog Beta-actin (pan Ab-5) | 42 | 1:2,000 | Neomarkers |