Figure 4

Restricted feeding (RF) effects on Rgs16 mRNA expression, body weight, and feeding. Top:Rgs16 mRNA expression in SCN (in situ) and liver (Northern, 20 μg/lane) of fasted (Z5) and fed (Z9) pair-caged female C57BL/6 mice. Zeitgeber time line, 12 hr light (open bar), 12 hr dark (filled bar). Solid line, normal chow ad libitum fed; dotted line, fasted; RF conditions, ad libitum fed only during Z5–Z9. Middle:changes in body weight before and after feeding during RF (avg 22 g at start of experiment, n = 46). Bottom: Day 0 is ad libitum fed mice, open bar indicates total food consumed per mouse per day; black bar indicates food consumed in first 30 min of the dark phase (7% of total), n = 12 pair-caged mice over 4 days. Day 1–4 (RF), open bar indicates total food consumed in 4 hr, Z5–Z9; black bar is food consumed in first 30 min (day 1, 31% of total; day 2-4, 38% of total; n = 46). Z, Zeitgeber time; RF, restricted feeding