The establishment and characterization of the first canine hepatocellular carcinoma cell line, which resembles human oncogenic expression patterns

Table 2 Oligonucleotides for quantitative RT-PCR used in this study.

Primer	Extension	Primer sequence (5'-3')	Tm	Product size (bp)	Accession Number
HPRT	U	AGCTTGCTGGTGAAAAGGAC	56	100	L77488/ L77489
	L	TTATAGTCAAGGGCATATCC
Beta-actin	U	GATATCGCCGCGCTCGTCGTC	55	350	Z70044
	L	GGCTGGGGTGTTGAAGGTCTC
c-MET	U	TGTGCTGTGAAATCCCTGAATAGAATC	58	112	AB118945
	L	CCAAGAGTGAGAGTACGTTTGGATGAC
PTEN	U	AGATGTTAGTGACAATGAACCT	64,5	110	U92435
	L	GTGATTTGTGTGTGCTGATC
P27Kip	U	CGGAGGGACGCCAAACAGG	59	90	AY455798
	L	GTCCCGGGTCAACTCTTCGTG
Bcl-2	U	TGGAGAGCGTCAACCGGGAGATGT	62	87	AB116145
	L	AGGTGTGCAGATGCCGGTTCAGGT
Beta-catenin	U	ATGGGTAGGGCAAATCAGTAAGAGGT	64	107	AY485996
	L	AAGCATCGTATCACAGCAGGTTAC
TGF-alpha	U	CCGCCTTGGTGGTGGTCTCC	63	122	AY458143
	L	AGGGCGCTGGGCTTCTCGT
SOCS3	U	ACACCAGCCTGCGCCTCAAGACCT	63	119	AY485997
	L	CGCCTCGCCGCCCGTCA
Collagen I	U	GTGTGTACAGAACGGCCTCA	61	111	AF056303
	L	TCGCAAATCACGTCATCG
ODC	U	GTGGGCGATTGGATGCTCTTTG	59	111	BI395954
	L	TGTTGGCCCCGACATCACATAGTAG