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Table 2 Liver levels of investigated parameters of Sprague-Dawley rats after in vivo treatment with nitric oxide donor (SNP) and peroxynitrite. Data expressed as Mean (SD); n = 8 per group.

From: Sodium nitroprusside and peroxynitrite effect on hepatic DNases: an in vitro and in vivostudy

Parameters Control SNP Peroxynitrite
Lipid peroxidation (MDA) (μmol/g protein) 54.42 (8.68) 52.5 (4.34) 58.2 (6.11)
SH groups (μmol/g protein) 636.04 (74.58) 538.67 (82.87) 505.52 (41.43)
NO2+NO3 (μmol/g protein) 6.15 (2.91) 6.04 (2.61) 5.74 (1.03)
Carbonyl groups (μmol/g protein) 4.14 (0.13) 4.25 (0.12) 4.20 (0.26)
Nitrotyrosine (nmol/g protein) 10.26 (2.62) 11.40 (1.24) 16.62 (3.58)
DNA (mg/g tissue) 10.71 (1.75) 12.65 (1.01) 12.15 (1.25)
DNA fragmentation (%) 11.99 (0.33) 13.21 (1.43) 13.0 (0.89)
Alkaline-DNase (U/g protein) 11.95 (0.57) 15.30 (0.72) 16.45 (1.04)
Acid-DNase (U/g protein) 15.28 (3.42) 12.34 (0.35) 9.34 (0.76)
  1. Male Sprague-Dawley rats three months old were allocated into three different groups. Either peroxynitrite (0.5 ml/kg BW of 30 mmol solution) or SNP (10 mg/kg BW) in a volume of 100 μl were administrated in bolus in systemic circulation. The control group received physiological saline solution at the same volume. All procedures were carried out as described in Methods.