Table 1 Conditions of enzyme assays.
From: Scavenger properties of cultivated pig liver endothelial cells
Enzyme | Conc. (mM) | Substrate | Buffer | pH | Incubation time (min) | Solubilisate volume (μl) |
|---|---|---|---|---|---|---|
α-Mannosidase | 2.5 | 4-methylumbelliferyl-α-D-mannopyranoside | Phosphate/citrate (0.1 M) | 4.0 | 120 | 40 |
Hexosaminidase | 5.0 | 4-methylumbelliferyl-N-acetyl-β-D-glucosaminide | Phosphate/citrate (0.1 M) | 4.5 | 30 | 5 10 (PC) |
Glucuronidase | 2.5 | 4-methylumbelliferyl-β-D-glucuronide | Acetate (0.1 M) | 4.5 | 120 | 20 5 (LSEC) |
Acid phosphatase | 1.0 | 4-methylumbelliferyl-phosphate | Acetate (0.1 M) | 4.5 | 30 | 10 |
Aryl sulphatase | 10.0 | 4-methylumbelliferyl-sulfate | Acetate (0.5 M) | 5.5 | 120 | 40 |
Acid lipase | 0.3 | 4-methylumbelliferyl-oleate | Acetate (0.1 M)* | 4.0 | 60 | 20 |