Figure 1

Liver sections were stained with H&E (A-C) or analyzed for nitrotyrosine (NT) protein adducts (D-F). Untreated controls (A,D) are compared to animals treated with 300 mg/kg acetaminophen (AAP) for 1 h (B,E) or 6 h (C,F). Controls (A) and 1 h AAP (B): The liver was histologically normal. 6 h AAP (C): Confluent areas of necrosis were seen around all centrilobular regions. Extensive hemorrhage was present. Control (D): No evidence of NT staining. 1 h AAP (E): NT staining localized in vascular lining cells. 6 h AAP (F): Confluent centrilobular NT staining present in hepatocytes.