Figure 2From: Lipopolysaccharide-mediated signal transduction: Stabilization of TNF-alpha mRNA contributes to increased lipopolysaccharide-stimulated TNF-alpha production by Kupffer cells after chronic ethanol feedingChronic ethanol exposure sensitizes RAW 264.7 macrophages to low concentrations of lipopolysaccharide. RAW 264.7 macrophages were cultured with and without 25 mM ethanol for 48 h and then stimulated with 0–5 ng/ml lipopolysaccharide (E. coli serotype O26:B6) in the presence of 10% serum for 60 min. RNA was isolated and quantity of TNF-alpha and GAPDH mRNA measured by ribonuclease protection assay.Back to article page