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Figure 2 | Comparative Hepatology

Figure 2

From: Lipopolysaccharide-mediated signal transduction: Stabilization of TNF-alpha mRNA contributes to increased lipopolysaccharide-stimulated TNF-alpha production by Kupffer cells after chronic ethanol feeding

Figure 2

Chronic ethanol exposure sensitizes RAW 264.7 macrophages to low concentrations of lipopolysaccharide. RAW 264.7 macrophages were cultured with and without 25 mM ethanol for 48 h and then stimulated with 0–5 ng/ml lipopolysaccharide (E. coli serotype O26:B6) in the presence of 10% serum for 60 min. RNA was isolated and quantity of TNF-alpha and GAPDH mRNA measured by ribonuclease protection assay.

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