Preface

The liver group in Tucson, Arizona, had great pleasure in hosting the 11th International Symposium on the Cells of the Hepatic Sinusoid and their Relation to Other Cells (ISCHS) from the 25th through 29th August, 2002.

In spite of the reduced travel resulting from the September 11, 2001 tragedy in New York City, we had a total participation by 102 delegates from around the world, representing Australia, Belgium, Brazil, England, Finland, France, Germany, Hungary, Italy, Japan, Netherlands, New Zealand, Norway, Poland, Spain, Sweden, Thailand, and the United States. In total, 6 tutorials, 36 platform presentations, and 37 poster presentations were made. I wish to thank all participants in the meeting, particularly those who gave lectures, papers, or presented posters, and especially those who also prepared their presentations for publication in these Proceedings. I also wish to thank the Scientific Organizing Committee (H. Jaeschke, M. Naito, Y. Shiratori, B. Smedsrød, F. Vidal-Vanaclocha, E. Wisse, R.S. McCuskey) for organizing the different scientific sessions and E. Rocha and E. Wisse for arranging the first electronic publication of the Proceedings of this series of Symposia. Finally, my special thanks to the dedicated work of S. Eastman and C. Martin for their extensive efforts in organizing both the Symposium and these Proceedings.

Final Remark

The Symposium was a great success and clearly highlighted the current status of research on sinusoidal lining cells as well as future directions. As a result, on behalf of the Scientific Organizing Committee, we hope that these Proceedings will encourage more research in the area of cells of the hepatic sinusoids and their interaction with other cells. We look forward to sharing new findings at the 12th ISCHS to be held in Bilbao, Spain in 2004 under the organization of Fernando Vidal-Vanaclocha and his colleagues.

Robert S. McCuskey

President of ISCHS

2000–2002

List of sessions and their contents

Opening Lecture: KARL WILHELM KUPFFER AND HIS CONTRIBUTIONS TO MODERN HEPATOLOGY

Kenjiro Wake

Session I: Stellate Cells in Fibrosis

Tutorial: MOLECULAR MECHANISM OF STELLATE CELL ACTIVATION AND THERAPEUTIC STRATEGY FOR LIVER FIBROSIS

Norifumi Kawada

3-D STRUCTURE OF EXTRACELLULAR MATRIX REGULATES GENE EXPRESSION IN CULTURED HEPATIC STELLATE CELLS TO INDUCE PROCESS ELONGATION

Sato M, Sato T, Kojima N, Imai K, Higashi N, Wang D-R, Senoo H

SIGNALS FOR HEPATIC FIBROGENESIS IN PEDIATRIC CHOLESTATIC LIVER DISEASE: REVIEW AND HYPOTHESIS

Ramm GA, Hoskins AC, Greco SA, Pereira TM, Lewindon PJ

THIOREDOXIN PREVENTS THIOACETAMIDE-INDUCED ACUTE HEPATITIS

Okuyama H, Shimahara Y, Nakamura H, Araya S, Kawada N, Yamaoka Y, Yodoi J

PPAR GAMMA AND HEPATIC STELLATE CELLS

Hazra S, Miyahara T, Rippe RA, Tsukamoto H

N-CADHERIN CLEAVAGE DURING ACTIVATED HEPATIC STELLATE CELL APOPTOSIS IS INHIBITED BY TISSUE INHIBITOR OF METALLOPROTEINASE-1

Murphy F, Waung J, Collins J, Arthur MJP, Nagase H, Mann D, Benyon RC, Iredale JP

AMINO ACIDS, L -CYSTEINE AND L -METHIONINE, ATTENUATE THE ACTIVATION OF RAT STELLATE CELLS IN PRIMARY CULTURE

Matsui H, Takashima T, Maeda N, Imanishi Y, Uyama N, Okuyama H, Kawada N

INVOLVEMENT OF GALECTIN-1 AND GALECTIN-3 IN PROLIFERATION AND MIGRATION OF RAT HEPATIC STELLATE CELLS IN CULTURE

Maeda N, Kawada N, Seki S, Ikeda K, Okuyama H, Hirabayashi J, Kasai K, Yoshizato K

SUPPRESSION OF RAT STELLATE CELL ACTIVATION AND LIVER FIBROSIS BY A JAPANESE HERBAL MEDICINE, INCHINKO-TO (TJ135)

Imanishi Y, Maeda N, Matsui H, Takashima T, Seki S, Arakawa T, Kawada N

EXPRESSION OF LEPTIN RECEPTORS IN HEPATIC SINUSOIDAL CELLS

Ikejima K, Lang T, Zhang Y-J, Yamashina S, Honda H, Yoshikawa M, Hirose M, Enomoto N, Kitamura T, Takei Y, Sato N

INTERCELLULAR ADHESIVE STRUCTURES BETWEEN STELLATE CELLS – AN ANALYSIS IN CULTURED HUMAN HEPATIC STELLATE CELLS

Imai K, Sato M, Sato T, Kojima N, Miura M, Higashi N, Wang D-R, Suzuki S, Senoo H

ACTIVATION OF STELLATE CELLS BEFORE INDUCTION OF HEPATIC FIBROSIS – PRECISE TIMING IN CHOLINE-DEFICIENT DIET-FED RAT MODEL

Besshi K, Fujiwara M, Senoo H, Kondou Y, Ohsugi Y, Hayashi T, Ishidate K

PERIPORTAL STELLATE CELLS IN SUBJECTS WITH CHRONIC HEPATITIS C WITH A VARIED SERUM ALANINE AMINOTRANSFERASE LEVEL

Fujiwara M, Besshi K, Takemura T, Itoh Y, Tsujino T, Minagawa N, Nakata R, Yoshitsugu M, Kato Y, Ihori M, Okayasu I, Senoo H, Wake K

INTRALOBULAR DISTRIBUTION OF VITAMIN A-STORING LIPID DROPLETS IN HEPATIC STELLATE CELLS WITH SPECIAL REFERENCE TO POLAR BEAR AND ARCTIC FOX

Higashi N, Imai K, Sato M, Sato T, Kojima N, Miura M, Wold HL, Moskaug J, Berg T, Norum KR, Roos N, Wake K, Blomhoff R, Senoo H

EXPRESSION OF THE PEROXISOME PROLIFERATOR-ACTIVATED RECEPTORS (PPARs) IN THE HEPATIC STELLATE CELLS

Sato T, Sato M, Miura M, Higashi N, Da-Ren W, Suzuki S, Imai K, Kojima N, Senoo H

DECREASED CAPACITY FOR VITAMIN A STORAGE IN HEPATIC STELLATE CELLS IN ARCTIC ANIMALS

Senoo H, Wake K, Wold H, Higashi N, Imai K, Moskaug JO, Kojima N, Miura M, Sato T, Sato M, Roos N, Berg T, Norum KR, Blomhoff R

5-LIPOXYGENASE (5-LO) IS INVOLVED IN KUPFFER CELL SURVIVAL. POSSIBLE ROLE OF 5-LO PRODUCTS IN THE PATHOGENESIS OF LIVER FIBROSIS

Titos E, Planagumà A, López-Parra M, Villamor N, Miquel R, Jiménez W, Arroyo V, Rivera F, Rodés J, Clària J

REGULATION OF MATRIX METALLOPROTEINASE EXPRESSION BY EXTRACELLULAR MATRIX COMPONENTS IN CULTURED HEPATIC STELLATE CELLS

Wang DR, Sato M, Sato T, Kojima N, Higashi N, Senoo H

HMG-COA REDUCTASE INHIBITOR MODULATES COLLAGEN GEL-CONTRACTION BY HEPATIC MYOFIBROBLAST-LIKE STELLATE CELL LINE: INVOLVEMENT OF GERANYLGERANYLATED PROTEINS

Yanase M, Ikeda H, Matsui A, Noiri E, Tomiya T, Arai M, Inoue Y, Tejima K, Nagashima K, Nishikawa T, Kimura S, Fujiwara K, Rojkind M, Ogata I

Session II: Functional Aspects of Sinusoidal Endothelial Cells

Tutorial: CLEARANCE FUNCTION OF SCAVENGER ENDOTHELIAL CELLS

Bård Smedsrød

FC GAMMA-RECEPTORS IN CELLS OF THE HEPATIC SINUSOID

Braathen H, Berg T, Mousavi SA, Kjeken R

THE LIVER SINUSOIDAL ENDOTHELIAL CELL HYALURONAN RECEPTOR AND ITS HOMOLOG, STABILIN-1 – THEIR ROLES (KNOWN AND UNKNOWN) IN ENDOCYTOSIS

McCourt PAG, Hansen B, Svistuonov D, Johansson S, Longati P, Schledzewski K, Kzhyshkowska J, Goerdt S, Johansson S, Smedsrød B

MALONDIALDEHYDE-ACETALDEHYDE (MAA) MODIFIED PROTEINS INDUCE PRO-INFLAMMATORY AND PRO-FIBROTIC RESPONSES BY LIVER ENDOTHELIAL CELLS

Thiele GM, Duryee MJ, Willis MS, Sorrell MF, Freeman TL, Tuma DJ, Klassen LW

PRELIMINARY ANALYSIS OF THE SINUSOIDAL ENDOTHELIUM AND SPACE OF DISSE IN AGEING PAPIO HAMADRAYAS

Cogger VC, Warren A, Fraser R, Ngu M, McLean AJ, Le Couteur DG

THE EFFECT OF CYTOCHALASIN B-LOADED LIPOSOMES ON THE ULTRASTRUCTURE OF THE LIVER SIEVE

Braet F, Vekemans K, Morselt H, De Zanger R, Wisse E, Scherphof G, Kamps J

STUDY OF THE REAPPEARANCE OF SIEVE PLATE-PORES IN IMMORTALIZED SINUSOIDAL ENDOTHELIAL CELLS: EFFECT OF ACTIN INHIBITOR IN MIXED PERFUSION CULTURES

Saito M, Matsuura T, Masaki T, Maehashi H, Braet F

Session III: Development and Regeneration

ROLE OF HEPATIC STELLATE CELLS IN THE EARLY PHASE OF LIVER REGENERATION IN RAT: FORMATION OF TIGHT ADHESIONS TO PARENCHYMAL CELLS

Mabuchi A, Mullaney I, Sheard PW, Hessian PA, Zimmermann A, Senoo H, Wheatley AM

IMAGING LIVER DEVELOPMENT/REMODELING IN THE SEE-THROUGH MEDAKA FISH

Hinton DE, Wakamatsu Y, Ozato K, Kashiwada S

Session IV: Non-Parenchymal Cells and Liver Injury

Tutorial: LIPOPOLYSACCHARIDE-MEDIATED SIGNAL TRANSDUCTION: STABILIZATION OF TNF ALPHA mRNA CONTRIBUTES TO INCREASED LIPOPOLYSACCHARIDE-STIMULATED TNF ALPHA PRODUCTION BY KUPFFER CELLS AFTER CHRONIC ETHANOL FEEDING

Kishore R, McMullen MR, Cocuzzi E, Nagy LE

IMMUNOMODULATORY ROLE OF KUPFFER CELL IN LIVER ALLOGRAFTS

Sun Z, Wada T, Hoshino S, Uchikura K, Klein AS

EFFECTS OF ACETAMINOPHEN ON HEPATIC MICROCIRCULATION IN MICE

Ito Y, Machen NW, Abril ER, McCuskey RS

FUNCTIONAL DIFFERENCES BETWEEN PERIPORTAL AND PERIVENOUS KUPFFER CELLS ISOLATED BY DIGITONIN-COLLAGENASE PERFUSION

Bykov I, Ylipaasto P, Eerola L, Lindros KO

THE REGULATORY ROLE OF PROSTAGLANDIN E ₂ IN LIVER (PATHO) PHYSIOLOGY IS CONTROLLED AT ITS SITE OF SYNTHESIS AND ITS ACTION ON THE RECEPTORS

Dieter P, Scheibe R, Bezugla Y, Matthé, Schuch S, Treffkorn L, Bernard B, Kamionka S, Kolada A

SIGNALING ROLE OF IRON IN NF KAPPA-B ACTIVATION IN HEPATIC MACROPHAGES

Xiong S, Hongyun S, Tsukamoto H

THALIDOMIDE PREVENTS ALCOHOLIC LIVER INJURY IN RATS THROUGH INHIBITION OF KUPFFER CELL SENSITIZATION

Enomoto N, Takei Y, Hirose M, Ikejima K, Kitamura T, Sato N

AN IN VIVO METHOD FOR DETERMINATION OF ENDOSOMAL DISTRIBUTION OF BOTH LIGAND AND ASIALOGYLCOPROTEIN RECEPTOR IN RAT LIVER

Dalton SR, Wiegert RL, Casey CA

ULTRASTRUCTURAL CHARACTERIZATION OF THE DEATH PROCESS OF HEPATOCYTES IN NEONATAL MOUSE LIVER

Sasaki K, Sonoda Y, Kumano I, Suda M

USE OF FLOW CYTOMETRIC ANALYSIS TO EXAMINE THE UPTAKE OF APOPTOTIC BODIES BY HEALTHY HEPATOCYTES

Casey C, Baldwin C, Kubik J, Hindemith A, McVicker B

CHEMILUMINESCENCE DETECTION OF REACTIVE OXYGEN SPECIES IN ISOLATED KUPFFER CELLS DURING PHAGOCYTOSIS OF TREPONEMA PALLIDUM

Aldini R, Marangoni A, Guardigli M, Sambri V, Giacani L, Montagnani M, Roda A, Cevenini R

HEME OXYGENASE-1 INDUCTION IN HEPATOCYTES AND NON-PARENCHYMAL CELLS PROTECTS AGAINST LIVER INJURY DURING ENDOXEMIA

Dorman RB, Bajt ML, Farhood A, Mayes J, Jaeschke H

BETA ₂ -GLYCOPROTEIN I INHIBITION OF MOUSE KUPFFER CELLS RESPIRATORY BURST DEPENDS ON LIVER ARCHITECTURE

Gomes LF, Knox PR, Simon-Giavarotti KA, Junqueira VBC, Sans J, Videla LA

ROLE OF AIM IN CORYNEBACTERIUM-INDUCED GRANULOMA FORMATION IN MICE

Kuwata K, Watanabe H, Yamamoto T, Miyazaki T, Naito M

PRECISION-CUT LIVER SLICES IN CULTURE AS A TOOL TO ASSESS THE PHYSIOLOGICAL INVOLVEMENT OF KUPFFER CELLS IN HEPATIC METABOLISM

Neyrinck AM, Gomez C, Delzenne NM

PEROXYNITRITE FORMATION AND SINUSOIDAL CELL INJURY DURING ACETAMINOPHEN HEPATOTOXICITY IN MICE

Knight TR, Jaeschke H

KUPFFER AND STELLATE CELL PROTEOGLYCANS MEDIATE MALARIA SPOROZOITE TARGETING TO THE LIVER

Pradel G, Garapaty S, Frevert U

CHLOROTYROSINE PROTEIN ADDUCTS ARE RELIABLE BIOMARKERS OF NEUTROPHIL-INDUCED CYTOTOXICITY IN VIVO

Gujral JS, Hinson JA, Jaeschke H

Session V: Role of Sinusoidal Cells in Tumor Progression

CYTOTOXIC REACTIONS OF CC531s TOWARDS LIVER SINUSOIDAL ENDOTHELIAL CELLS: A MICROSCOPICAL STUDY

Vekemans K, Timmers M, Vermijlen D, De Zanger R, Wisse E, Braet F

CONFOCAL LASER SCANNING MICROSCOPIC STUDY OF THE KILLING OF METASTATIC COLON CARCINOMA CELLS BY KUPFFER CELLS IN THE EARLY ONSET OF HEPATIC METASTASIS

Timmers M, Vekemans K, Vermijlen D, Wisse E, Braet F

ELEVATED LEVELS OF TISSUE INHIBITOR OF METALLOPROTEINASES (TIMPS) IN HUMAN HEPATOCELLULAR CARCINOMAS

Matsumoto E, Nakatsukasa H, Nouso K, Nakamura S, Suzuki M, Kobayashi Y, Uemura M, Sato S, Yumoto E, Yokoyama J, Tsuboi S, Tanaka H, Takuma Y, Fujikawa T, Shiratori Y

EXPRESSION OF GROWTH FACTORS IN COLORECTAL CARCINOMA LIVER METASTATIC PATIENTS AFTER PARTIAL HEPATECTOMY: IMPLICATIONS FOR A FUNCTIONAL ROLE IN CELL PROLIFERATION DURING LIVER REGENERATION

Lukomska B, Dluzniewska J, Polanski J, Zajac L

MIGRATION OF LIVER SINUSOIDAL LEUKOCYTES TO THE LIVER COLON ADENOCARCINOMA METASTASES

Durowicz S, Lukomska B, Dluzniewska J, Laszuk D, Olszewski WL

REDUCTION OF LIVER METASTASES OUTGROWTH BY TUMOUR ANTIGEN-PULSED DENDRITIC CELL VACCINATION

Mels AK, Mayen I, van Egmond M, Beelen RHJ, Meijer S, Richters CD

CORRELATION BETWEEN IN VIVO ACCUMULATION AND IN VITRO ADHESION OF LIVER ASSOCIATED LYMPHOCYTES IN AND AROUND LIVER ADENOCARCINOMA METASTASES

Stanczyk M, Olszewski WL, Durowicz S, Maruszynski M

HISTOPATHOLOGICAL CHARACTERIZATION OF HEPATOCELLULAR CARCINOMAS WHICH ARE UNDETECTED BY DYNAMIC COMPUTED TOMOGRAPHY

Yamamoto T, Hirohashi K, Sakabe K, Shuto T, Uenishi M, Ogawa M, Tanaka S, Tanaka H, Kubo S, Kaneda K, Sakurai M

EXPRESSION OF FIBRILLIN-1 IN FOCAL NODULAR HYPERPLASIA OF THE LIVER: A ROLE IN MICROCIRCULATION ADAPTABILITY

Lepreux S, Desmouliére A, Rosenbaum J, Balabaud C, Bioulac-Sage P

PIT CELLS EXCLUSIVELY KILL P815 TUMOR CELLS BY THE PERFORIN/GRANZYME PATHWAY

Vermijlen D, Luo D, Froelich CJ, Medema JP, Alain J, Kummer JA, Willems E, Braet F, Wisse E