Treatments
|
Hours
|
---|
|
6
|
8
|
16
|
24
|
48
|
---|
Control
|
-
|
-
|
-
|
±
|
±
|
Pb(NO3)2
|
-
|
-
|
-
|
±
|
+++
|
Pb(C2H3O2)2
|
-
|
-
|
±
|
+++
|
++++
|
KNO3
|
-
|
-
|
-
|
±
|
±
|
C.M.
|
-
|
-
|
-
|
Apoptosis (35%)
|
Apoptosis (48%)
|
- * Control animals were injected with saline. The presence of necrosis is represented by +: ± <20%; + 20–40%; ++ 40–60%; +++ 60–80%; ++++ >80%. C.M. = conditioned medium collected from Kupffer cell cultures incubated for 24 h with Pb(NO3)2 (10 mM). Apoptosis was detected on slides of haematoxylin-eosin stained cells, counting at least 300 cells in at least 10 randomly selected fields.