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Table 5 Evaluation of apoptosis versus necrosis in hepatocyte cultures incubated with 10 mM Pb(NO3)2, Pb(C2H3O2)2 or KNO3 and with CM from Kupffer cells.*

From: Kupffer cells promote lead nitrate-induced hepatocyte apoptosis via oxidative stress

Treatments Hours
  6 8 16 24 48
Control - - - ± ±
Pb(NO3)2 - - - ± +++
Pb(C2H3O2)2 - - ± +++ ++++
KNO3 - - - ± ±
C.M. - - - Apoptosis (35%) Apoptosis (48%)
  1. * Control animals were injected with saline. The presence of necrosis is represented by +: ± <20%; + 20–40%; ++ 40–60%; +++ 60–80%; ++++ >80%. C.M. = conditioned medium collected from Kupffer cell cultures incubated for 24 h with Pb(NO3)2 (10 mM). Apoptosis was detected on slides of haematoxylin-eosin stained cells, counting at least 300 cells in at least 10 randomly selected fields.