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Figure 2 | Comparative Hepatology

Figure 2

From: Eggshell and egg yolk proteins in fish: hepatic proteins for the next generation: oogenetic, population, and evolutionary implications of endocrine disruption

Figure 2

Immunohistochemical staining of a cod (Gadus morhua) ovarian follicle with oocyte, probed with rabbit antiserum to cod zona radiata proteins. The zona radiata proteins (Zr) and the yolk (Y) protein vitellogenin are both synthesized in the liver of most fish species and transported to the ovary. (A) Section of whole oocyte, demonstrating specific immunohistochemical staining of the zona radiata, with no cross-reaction to yolk material (Y). (B) Higher magnification of the cod follicle. Zr denotes the zona radiata (positively stained). The follicle cells (theca, T, and granulosa, G) are indicated with arrowheads. Spherical bodies represent unstained yolk granules. Reproduced from Oppen-Berntsen et al. [19], with permission from University of the Basque Country Press (UBC Press) and the author.

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Comparative Hepatology

ISSN: 1476-5926