Characterization of chronic HCV infection-induced apoptosis

Comparative Hepatology

Table 3 Changes in apoptotic and pre apoptotic genes expression in HCV infected HepG2 cell line in vitro.

	Qualitative/Quantitative PCR (copy number/ml)		Apoptotic gene
Days	Cell free media	Cell lysate	Bcl-xL	Bcl-2	Bak	Fas	FasL
Day1	Positive/785	Positive	-	+	+++	++	-
Day2	Positive/Negative	Positive	-	+	+	++	-
Day3	Negative/Negative	Positive	-	+	++	++	-
Day7	Positive/13005	Positive	-	+	+	-	-
Day14	Positive/Negative	Positive	-	+	+	-	-
Day21	Negative/6782	Positive	-	+	-	-	+
Day28	Negative/24678	Positive	+	+	++	-	+
Day35	Positive/8892	Negative	-	+	-	-	+
Day45	Positive/Negative	Positive	+	+	-	-	++
Day52	Positive/7374	Negative	-	+	-	-	+++
Day59	Positive/22963	Positive	+	+	++	-	+++
HepG2 Control	-	-	-	+	+	+	-

+: Equal to the expression level in the HepG2; ++: twofold increase in the expression level; +++ threefold increase in the expression level.