A prospective assessment of the inter-laboratory variability of biochemical markers of fibrosis (FibroTest) and activity (ActiTest) in patients with chronic liver disease

Halfon, Philippe; Imbert-Bismut, Françoise; Messous, Djamila; Antoniotti, Gilles; Benchetrit, Didier; Cart-Lamy, Philippe; Delaporte, Gilles; Doutheau, Danièle; Klump, Théo; Sala, Michel; Thibaud, Didier; Trepo, Elisabeth; Thabut, Dominique; Myers, Robert P; Poynard, Thierry

doi:10.1186/1476-5926-1-3

Table 4 Laboratory analyzers and biochemical methods

From: A prospective assessment of the inter-laboratory variability of biochemical markers of fibrosis (FibroTest) and activity (ActiTest) in patients with chronic liver disease

	LAB 1 (Reference center)	LAB 2	LAB 3
α₂-macroglobulin
Analyzer	BN2	BN2	BN2
Method	Nephelemetry	Nephelemetry	Nephelemetry
Haptoglobin
Analyzer	BN2	BN2	BN2
Method	Nephelemetry	Nephelemetry	Nephelemetry
Apolipoprotein A1
Analyzer	BN2	RXL	RXL
Method	Nephelemetry	Turbidimetry	Turbidimetry
Total bilirubin
Analyzer	Modular	RXL	RXL
Method	Diazoreaction	Diazoreaction	Diazoreaction
Aminotransferase
Analyzer	Modular	RXL	RXL
Method	IFCC Pyridoxal	IFCC Pyridoxal	IFCC Pyridoxal
γ-glutamyl transpeptidase
Analyzer	Modular	RXL	RXL
Method	Szasz standardized	Szasz	Szasz
	LAB 4	LAB 5	LAB 6
α ₂ -macroglobulin
Analyzer	BN2	BN2	BN2
Method	Nephelemetry	Nephelemetry	Nephelemetry
Haptoglobin
Analyzer	BN2	Advia1650	Integra 800
Method	Nephelemetry	Turbidimetry	Turbidimetry
Apolipoprotein A1
Analyzer	Hitachi 911	Advia 1650	Integra 800
Method	Turbidimetry	Turbidimetry	Turbidimetry
Total bilirubin
Analyzer	Hitachi 911	Advia 1650	Integra 800
Method	Diazoreaction	Diazoreaction	Diazoreaction
Aminotransferase
Analyzer	Hitachi 911	Advia1650	Integra 800
Method	IFCC Pyridoxal	IFCC	IFCC
γ-glutamyl transpeptidase
Analyzer	Hitachi 911	Advia1650	Integra 800
Method	Szasz standardized	Szasz	Szasz standardized
	LAB 7	LAB 10	LAB 11
α ₂ -macroglobulin
Analyzer	BN2	BN2	Immage
Method	Nephelemetry	Nephelemetry	Nephelemetry
Haptoglobin
Analyzer	BN2	BN2	Immage
Method	Nephelemetry	Nephelemetry	Nephelemetry
Apolipoprotein A1
Analyzer	Advia 1650	CX5	Immage
Method	Turbidimetry	Turbidimetry	Nephelemetry
Total bilirubin
Analyzer	Advia	CX7	Vitros 250
Method	Diazoreaction	Diazoreaction	Diazoreaction
Aminotransferase
Analyzer	Advia	CX7	Vitros 250
Method	IFCC	IFCC	IFCC Pyrydoxal
γ-glutamyl transpeptidase
Analyzer	Advia 1650	CX7	Vitros 250
Method	Szasz	Szasz	Szasz

BN2, RXL, Vitros 250: Dade Behring, Marburg, Germany. Hitachi 911, Integra 800: Modular DP Roche Diagnostics, Mannheim, Germany. Immage, CX5, CX7: Beckman Coulter, Brea, California, USA. Advia 1650: Bayer Diagnostics, Tarrytown, New Jersey, USA. IFCC: International Federation of Clinical Chemistry.

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Comparative Hepatology