Table 1 Antibodies.
From: Response of sinusoidal mouse liver cells to choline-deficient ethionine-supplemented diet
Antibody | Supplier/source | Dilution |
|---|---|---|
Rat-anti-mouse CD31(PECAM-1) | BD Pharmingen | 1:100 |
Rat-anti-mouse F4/80 (Clone A3-1) | Serotech | 1:50 |
Rabbit-anti-cow-cytokeratin | DAKO | 1:500 |
Rabbit-anti-cow-GFAP | DAKO | 1:500 |
Goat-anti-rabbit-pyruvate kinase | Rockland incorporation | 1:500-1:1,000 |
Mouse-anti-human pyruvate kinase (Clone DF 4) | Schebo Biotech AG | 1:50 |
Rabbit-anti-human-M2-Pk | Cell Signaling | 1:100 |
Chicken-anti-vimentin | Chemicon | 1:5,000 |
Mouse-anti-vimentin S82 | 1:100 | |
Rat-anti-BrdU | Serotech | 1:50 |
Mouse-anti-human-E-cadherin | BD Transduction laboratories | 1:100 |
Mouse-anti-rat-Nestin (Clone Rat-401) | Chemicon | 1:100 |
Anti-alpha-smooth muscle actin (Clone 1A4) | SIGMA | 1:100 |
Mouse-anti-human-N-cadherin | BD Transduction laboratories | 1:100 |
Rabbit-anti-mouse-LI-cadherin | Gift from Dr. R. Geßner | 1:1,000 |