Histological alterations in livers from transgenic and non-transgenic mice injected with CFSE-labeled splenocytes from immunized mice. A) Immunofluorescent analysis of frozen liver sections (5 μm thick) of a transgenic mouse showing CFSE labeled cells scattered over all the liver section. The fluorescent cells are indicated by arrows. B) H&E stained liver section of transgenic mouse showing steatosis. There is infiltration of lymphocytes in the liver which is concentrated close to hepatic steatosis (indicated by arrows). C) Immunofluorescence analysis of frozen liver sections (5 μm thick) of non-transgenic mouse showing no CFSE labeled cells over the liver section. D) H&E staining of liver section of non-transgenic mouse showing normal histology of the liver and no lymphocyte infiltration. Scale bar = 50 μm.