Daytime food restriction alters liver glycogen, triacylglycerols, and cell size. A histochemical, morphometric, and ultrastructural study

Table 1 Change of body weight (BW) of rats after 3 weeks under restricted feeding schedules.

Treatment	Initial BW (g)	Final BW (g)	Δ BW (%)
*Food ad libitum*
08:00 h	151 ± 3	320 ± 21	169 (112%)
11:00 h	150 ± 2	329 ± 26	179 (119%)
14:00 h	153 ± 2	337 ± 31	184 (120%)
*Food restricted schedule*
08:00 h	150 ± 2	182 ± 17*	32 (21%)*
11:00 h	151 ± 3	192 ± 20*	41 (27%)*
14:00 h	149 ± 1	246 ± 23* ⁺	97 (65%)* ⁺
*24 h Fasting*
11:00 h	321 ± 4	298 ± 3	-23 (-7%)

Values are means ± SE for 6 independent observations. Male Wistar rats were under food restriction for three weeks. Food access from 12:00 to 14:00 h. Control groups included rats fed ad libitum and rats fasted for 24 h. Results are expressed as mean ± SEM of 6 independent determinations. Significant difference between RFS and ad-libitum groups (*), within the same experimental group (+), and different from 24-h fasting group (x). Differences derived from to Tukey's post hoc test (α = 0.05).