Table 3 Used antibodies with manufacturer and methods
Antibody | Manufacturer | Type | Clone | Antigen Retrieval | Dilution | Wash Buffer | Incubation |
|---|---|---|---|---|---|---|---|
Keratin 19 | Novocastra Laboratories Ltd. | mouse monoclonal | B170 | Prot K | 1:100 | TBS | 1 hr RT |
Keratin 7 | Dakocytomation | mouse monoclonal | OV-TL 12/30 | Prot K | 1:25 | TBS | O/N 4°C |
HepPar-1 | Dakocytomation | mouse monoclonal | OCH 1E5 | Tris-EDTA | 1:50 | PBS | O/N 4°C |
Glypican-3 | BioMosaics | mouse monoclonal | 1G12 | Citrate | 1:100 | PBS | O/N 4°C |