Expression of plcβ4 in mouse liver tissue. (A) PCR results using plcb4 set A primers and template cDNA obtained from RT-PCR of mouse liver total RNA. Lane 1: 1 kB DNA Ladder Plus (Invitrogen). Lane 2: 50 μl PCR reaction indicating plcβ4 product (326 bp). (B) Representative gels of plcβ4 gene and gapdh expression over the course of the day in mice housed in LD (left) and DD (right). Each lane represents data from an individual mouse sacrificed at the time of day indicated above each lane. Relative amounts of plcβ4 gene expression across the 24-hour day of mice housed in Light:Dark (C) or Dark:Dark (D). Normalized data represent mean ± SE of 3 mice at each time point. Cosinor-fitted curves have been drawn. The black bars at the bottom of each graph represent the period of darkness.