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Table 2 Lactate and glucose measurements in supernatants obtained from dishes with or without cells.

From: The influence of oxygen tension on the structure and function of isolated liver sinusoidal endothelial cells

Culture Supernatants

Time (h)

Lactate (mmol/L/106 cells)

Glucose (mmol/L/106 cells)

Cell-free (20% O2)

0–24

0.10 (± 0.20)

11.81 (± 0.24)

LSECs (20% O2)

0–24

1.19 (± 0.42)

10.96 (± 0.40)

LSECs (5% O2)

0–24

2.77 (± 0.59)

10.83 (± 0.20)

LSECs (20% O2)

24–48

1.31 (± 0.38)

10.70 (± 0.20)

LSECs (5% O2)

24–48

3.07 (± 1.12)

10.36 (± 0.20)

  1. Conditioned media collected from cell free incubations, or LSEC cultures were analysed for glucose and lactate. Values are means of triplicate measurements. The results are representative data obtained from three independent experiments.
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