Production of nitric oxide (NO) and hydrogen peroxide (H
) by LSEC. Secretion of nitric oxide (NO) was measured in LSEC culture supernatants by Griess reaction at 24 and 48 h (a). Culture media was collected from hyperoxic conditions (open bars) or normoxic conditions (filled bars) at 24 hours intervals. Final concentrations were estimated from individual standard curves. Generation of endogenous H2O2 was monitored in separate experiments at the indicated time-points in LSEC cultures by H2O2-mediated oxidation of DCFH-DA into DFC during 6 h (b). Values are total fluorescence emitted at 545 nm.