Figure 1From: The influence of oxygen tension on the structure and function of isolated liver sinusoidal endothelial cellsMorphological examination of LSEC cultures over time by light microscopy. Freshly isolated LSECs cultures were established on 24 well plates and incubated either at hyperoxia (a-c) or at normoxia (d-f). The general morphology of the cultures was monitored by light microscopy at day 1 (a, d), day 3 (b, d) and day 5 (c, f) after isolation. Decline of LSECs cultures may be observed in dishes maintained at atmospheric oxygen levels (a-c) after several days of culture.Back to article page