Table 1 Nucleotide Sequences of Dog-Specific Primers for Real-Time Quantitative PCR
From: Major HGF-mediated regenerative pathways are similarly affected in human and canine cirrhosis
Gene | Primer | Sequence (5'-3') | Tm (°C) | Product size (bp) | Accession number |
|---|---|---|---|---|---|
GAPDH | Forward | TGT CCC CAC CCC CAA TGT ATC | 58 | 100 | AB038240 |
| Â | Reversed | CTC CGA TGC CTG CTT CAC TAC CTT | Â | Â | Â |
HPRT | Forward | AGC TTG CTG GTG AAA AGG AC | 56 | 100 | L77488 / |
| Â | Reversed | TTA TAG TCA AGG GCA TAT CC | Â | Â | L77489 |
HGF | Forward | AAA GGA GAT GAG AAA CGC AAA CAG | 58 | 92 | BD105535 |
| Â | Reversed | GGC CTA GCA AGC TTC AGT AAT ACC | Â | Â | Â |
c-MET | Forward | TGT GCT GTG AAA TCC CTG AAT AGA AATC | 59 | 112 | AB118945 |
| Â | Reversed | CCA AGA GTG AGA GTA CGT TTG GAT GAC | Â | Â | Â |