Interactions Between Estrogen- and Ah-Receptor Signalling Pathways in Primary Culture of Salmon Hepatocytes Exposed to Nonylphenol and 3,3',4,4'-Tetrachlorobiphenyl (Congener 77)

Table 1 Primer pair sequences, accession numbers, amplicon size and annealing temperature conditions for genes of interest used for real-time PCR.

Target Gene	Primer sequence*		Amplicon size (nucleotides)	Annealing temperature (°C)	GenBank accession number
	Forward	Reverse
ERα	TCCAGGAGCTGTCTCTCCAT	GATCTCAGCCATACCCTCCA	173	55	DQ009007
ERβ	GAGCATCCAAGGTCACAATG	CACTTTGTCATGCCCACTTC	126	59	AY508959
Vtg	AAGCCACCTCCAATGTCATC	GGGAGTCTGTCCCAAGACAA	391	57	DY802177
Zr-protein	TGACGAAGGTCCTCAGGG	AGGGTTTGGGGTTGTGGT	113	55	AF407574
Vigilin	GGGATACGCACAGACACCTT	CCCAGATTCCACAGACACCT	86	60	DY802195
AhRα	AGGGGCGTCTGAAGTTCC	GTGAACAGGCCCAACCTG	82	60	AY219864
AhRβ	GACCCCCAGGACCAGAGT	GTTGTCCTGGATGACGGC	96	65	AY219865
AhRR	TTCCTCCAGGGACAGAAGAA	ATGGAGGGCAGCAGAAGAG	98	60	DQ372978
Arnt	AGAGCAATCCCAGGGTCC	TGGGAGGGTGATTGAGGA	107	60	DQ367887
CYP1A1	GAGTTTGGGCAGGTGGTG	TGGTGCGGTTTGGTAGGT	76	60	AF364076
UDPGT	ATAAGGACCGTCCCATCGAG	ATCCAGTTGAGGTCGTGAGC	113	55	DY802180
Proteasome	TCTTTGACCAGGTTGCACAG	CATACAAAGCTGGTGGCTCA	134	60	DY802110