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Table 1 Details concerning the used antibodies.

From: Morphological characterisation of portal myofibroblasts and hepatic stellate cells in the normal dog liver

Antibody

Manufacturer

Catalogue no.

Type

Clone

Dilution

Fixative

α-SMA

BioGenex

MU 128-UC

mouse monoclonal

clone1A4

1:1200

formalin

Desmin

Eurodiagnostica

2203PDE

rabbit polyclonal

-

1:80

formalin

GFAP

ICN Biomedicals

10555

rabbit polyclonal

-

1:80

formalin

GFAP

Biogenex

 

mouse monoclonal

-

1:40

frozen

Muscle actin

Dako

M0635

mouse monoclonal

HHF35

1:300

formalin

NCAM

Chemicon

 

rabbit polyclonal

 

1:50

frozen

Vimentin

Dako

M0776

mouse monoclonal

SY38

1:100

formalin

Synaptophysin

Dako

 

rabbit polyclonal

-

1:50

frozen

Vimentin

Biogenex

MU074-UC

mouse monoclonal

V9

1:150

formalin