Figure 3

α-SMA. Normal canine liver, stained with α-SMA antibody. a) Portal areas show positivity around the bile ducts, in the arterial tunica media, and in the wall of the portal veins. There is slightly irregular moderate staining in the perisinusoidal spaces throughout the parenchyma. b) HSC stain positive, producing a thin irregular positive band lining the sinusoids. c) HSC stain positive. A positive cell containing one large vacuole (arrow-head is placed in vacuole) and a dislocated nucleus is seen. d) In the portal area there is strong positivity around the bile ducts and in the arterial tunica media, and moderate positivity in the wall of the portal veins, while endothelial cells remain negative (horizontal arrow). A portal MF with moderate positivity (vertical arrow) is present.