Table 1 Primer sequences and other PCR analysis related details.
Gene | Primer | Tm (°C) | Number of cycles | Product size (bp) |
|---|---|---|---|---|
AFP1 | 5'-GCCCAGCATACGAAGAAAACA-3' 5'-TCTCTTTGTCTGGAAGCATTCCT-3' | 60 | 40 | 179 |
AFP2 | 5'-ACTACTTACAAAATCTGTTCCTCATTGG-3' 5'-ATGTAAATGTCGGCCAGTCC-3' | 60 | 40 | 169 |
Rev-Erb A | 5'-CCATGTTTGACTTCAGCGAGAA-3' 5'-AAGTACCACTGCCGTGAAAAGG-3' | 60 | 40 | 80 |
Rev-Erb B | 5'-GAGCATGCCACCCCATAGAG-3' 5'-TATTGCTCCACATTCCTGGGA-3' | 60 | 40 | 51 |
N-CoR | 5'-TCTGCTTTGTCGTCCACACC-3' 5'-GCTGTAGCGACTTGACGGTTTA-3' | 60 | 40 | 51 |
β-actin | 5'-GAAATCGTGCGTGACATTAAAGAG-3' 5'-GCGGCAGTGGCCATCTC-3' | 60 | 40 | 74 |