Table 2 Used antibodies in Western blot experiments.
Antigen | Product Size (kDa) | Dilution | Manufacturer | Secondary antibody | Dilution |
|---|---|---|---|---|---|
HGF | 82 | 1:100 | Neomarkers | Anti-mouse HRP | 1:20,000 |
p-c-MET (Tyr 1230/1234/1235) | 145 | 1:750 | Abcam | Anti-rabbit HRP | 1:20,000 |
c-MET | 145 | 1:750 | Sigma | Anti-goat HRP | 1:20,000 |
p-PKB (Thr 308) | 60 | 1:1,000 | Cell-Signaling | Anti-mouse HRP | 1:20,000 |
PKB | 60 | 1:250 | BD Biosciences | Anti-mouse HRP | 1:20,000 |
p-STAT3 (Ser 727) | 86 | 1:1,000 | Cell Signalling | Anti-rabbit HRP | 1:20,000 |
STAT3 | 86 | 1:2,500 | BD Biosciences | Anti-rabbit HRP | 1:20,000 |
p-Erk1/2 (Thr 202/Tyr 204) | 42/44 | 1:1,500 | Cell Signalling | Anti-rabbit HRP | 1:20,000 |
ERK1/2 | 42/44 | 1:1,000 | Cell Signalling | Anti-rabbit HRP | 1:20,000 |
TGF-β1 | 25 | 1:1,000 | Abcam | Anti-rabbit HRP | 1:20,000 |
p-Smad2 (Ser 465/467) | 58 | 1:2,000 | Cell-Signaling | Anti-rabbit HRP | 1:20,000 |
Smad2 | 58 | 1:500 | BD Biosciences | Anti-mouse HRP | 1:20,000 |
Collagen I | 95/210 | 1:500 | Calbiochem | Anti-mouse HRP | 1:20,000 |
Caspase-3 | 34/20/18 | 1:1,000 | Calbiochem | Anti-rabbit HRP | 1:20,000 |
Beta-actin (pan Ab-5) | 42 | 1:2,000 | Neomarkers | Anti-mouse HRP | 1:20,000 |