Regenerative and fibrotic pathways in canine hepatic portosystemic shunt and portal vein hypoplasia, new models for clinical hepatocyte growth factor treatment

Spee, Bart; Penning, Louis C; van den Ingh, Ted SGAM; Arends, Brigitte; IJzer, Jooske; van Sluijs, Frederik J; Rothuizen, Jan

doi:10.1186/1476-5926-4-7

Table 1 Nucleotide Sequences of Dog-Specific Primers for Real-Time Q-PCR.

From: Regenerative and fibrotic pathways in canine hepatic portosystemic shunt and portal vein hypoplasia, new models for clinical hepatocyte growth factor treatment

Gene	Primer	Sequence (5'-3')	°C	Product size (bp)	Accession number
GAPDH	Forward	TGT CCC CAC CCC CAA TGT ATC	58	100	AB038240
	Reversed	CTC CGA TGC CTG CTT CAC TAC CTT
HPRT	Forward	AGC TTG CTG GTG AAA AGG AC	56	100	L77488/L77489
	Reversed	TTA TAG TCA AGG GCA TAT CC
HGF	Forward	AAA GGA GAT GAG AAA CGC AAA CAG	58	92	BD105535
	Reversed	GGC CTA GCA AGC TTC AGT AAT ACC
c-MET	Forward	TGT GCT GTG AAA TCC CTG AAT AGA AATC	59	112	AB118945
	Reversed	CCA AGA GTG AGA GTA CGT TTG GAT GAC
TGFα	Forward	CCG CCT TGG TGG TGG TCT CC	63	136	AY458143
	Reversed	AGG GCG CTG GGC TTC TCG T
HGF activator	Forward	ACA CAG ACG TTT GGC ATC GAG AAG TAT	60	128	AY458142
	Reversed	AAA CTG GAG CGG ATG GCA CAG
p27kip	Forward	CGG AGG GAC GCC AAA CAG G	60	90	AY455798
	Reversed	GTC CCG GGT CAA CTC TTC GTG
TGF-β1	Forward	CAA GGA TCT GGG CTG GAA GTG GA	66	113	L34956
	Reversed	CCA GGA CCT TGC TGT ACT GCG TGT
TGF-β1 R I	Forward	CAG TCA CCG AGA CCA CAG ACA AAG T	59	101	AY455799
	Reversed	TGA AGA TGG TGC ACA AAC AAA TGG
TGF-β1 R II	Forward	GAC CTG CTG CCT GTG TGA CTT TG	61	116	AY455800
	Reversed	GGA CTT CGG GAG CCA TGT ATC TTG
UPA	Forward	CTG GGG AGA TGA AGT TTG AGG TGG	64.5	105	AY455801
	Reversed	TGG AAC GGA TCT TCA GCA AGG C
Bcl-2	Forward	TGG AGA GCG TCA ACC GGG AGA TGT	61	87	AB116145
	Reversed	AGG TGT GCA GAT GCC GGT TCA GGT
Fas Ligand	Forward	GGG GTC AGT CCT GCA ACA ACA A	54	94	AY603042
	Reversed	ATC TTC CCC TCC ATC AGC ATC AG
Caspase-3	Forward	ATC ACT GAA GAT GGA TGG GTT GGT	58	140	AB085580
	Reversed	GAA AGG AGC ATG TTC TGA AGT AGC ACT
HIF1α	Forward	TTA CGT TCC TTC GAT CAG TTG TCA	61	106	AY455802
	Reversed	GAG GAG GTT CTT GCA TTG GAG TC
Collagen I	Forward	GTG TGT ACA GAA CGG CCT CA	61	111	AF056303
	Reversed	TCG CAA ATC ACG TCA TCG
Collagen III	Forward	ATA GAG GCT TTG ATG GAC GAA	65	134	AB042266
	Reversed	CCT CGC TCA CCA GGA GC
Collagen IV	Forward	CAC AGC CAG ACA ACA GAT GC	67	151	U07888
	Reversed	GCA TGG TAC TGA AGC GAC G
Fibronectin	Forward	AGG TTG TTA CCA TGG GCA	61	91	U52106
	Reversed	GCA TAA TGG GAA ACC GTG TAG

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Comparative Hepatology