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Table 2 Nucleotide Sequences of Dog-Specific Primers for Quantitative Real-Time PCR

From: Differential expression of copper-associated and oxidative stress related proteins in a new variant of copper toxicosis in Doberman pinschers

Gene Primer Sequence (5'-3') Tm (°C) Product size (bp) Accession number
GAPDH Forward TGT CCC CAC CCC CAA TGT ATC 58 100 AB038240
  Reversed CTC CGA TGC CTG CTT CAC TAC CTT    
HPRT Forward AGC TTG CTG GTG AAA AGG AC 56 100 L77488 /
  Reversed TTA TAG TCA AGG GCA TAT CC    L77489
SOD1 Forward TGG TGG TCC ACG AGA AAC GAG ATG 64 99 AF346417
  Reversed CAA TGA CAC CAC AAG CCA AAC GAC T    
CAT Forward TGA GCC CAG CCC TGA CAA AAT G 62 119 AB012918
  Reversed CTC GAG CCC GGA AAG GAC AGT T    
GSS Forward CTG GAG CGG CTG AAG GAC A 62 131 AY572226
  Reversed AGC TCT GAG ATG CAC TGG ACA    
GPX1 Forward GCA ACC AGT TCG GGC ATC AG 62 123 AY572225
  Reversed CGT TCA CCT CGC ACT TCT CAA AA    
CCS Forward TGT GGC ATC ATC GCA CGC TCT G 64 96 AY572228
  Reversed GGG CCG GCC TCG CTC CTC    
p27KIP Forward CGG AGG GAC GCC AAA CAG G 60 90 AY455798
  Reversed GTC CCG GGT CAA CTC TTC GTG    
Bcl-2 Forward TGG AGA GCG TCA ACC GGG AGA TGT 61 87 AB116145
  Reversed AGG TGT GCA GAT GCC GGT TCA GGT    
ATOX1 Forward ACG CGG TCA GTC GGG TGC TC 67 137 AF179715
  Reversed AAC GGC CTT TCC TGT TTT CTC CAG    
COX17 Forward ATC ATT GAG AAA GGA GAG GAG CAC 60 127 AY603041
  Reversed TTC ATT CTT CAA GGA TTA TTC ATT TAC A    
ATP7A Forward CTA CTG TCT GAT AAA CGG TCC CTA AA 50 99 AY603040
  Reversed TGT GGT GTC ATC ATC TTC CCT GTA    
ATP7B Forward GGT GGC CAT CGA CGG TGT GC 56 136 AY603039
  Reversed CGT CTT GCG GTT GTC TCC TGT GAT    
CP Forward AAT TCT CCC TTC TGT TTT TGG TT 62 97 AY572227
  Reversed TTG TTT ACT TTC TCA GGG TGG TTA    
MT1A Forward AGC TGC TGT GCC TGA TGT G 64 130 D84397
  Reversed TAT ACA AAC GGG AAT GTA GAA AAC    
MURR1 Forward GAC CAA GCT GCT GTC ATT TCC AA 58 122 AY047597
  Reversed TTG CCG TCA ACT CTC CAA CTC A    
XIAP Forward ACT ATG TAT CAC TTG AGG CTC TGG TTT C 54 80 AY603038
  Reversed AGT CTG GCT TGA TTC ATC TTG TGT ATG    
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Comparative Hepatology

ISSN: 1476-5926