Differential expression of copper-associated and oxidative stress related proteins in a new variant of copper toxicosis in Doberman pinschers

Spee, Bart; Mandigers, Paul JJ; Arends, Brigitte; Bode, Peter; van den Ingh, Ted SGAM; Hoffmann, Gaby; Rothuizen, Jan; Penning, Louis C

doi:10.1186/1476-5926-4-3

Table 2 Nucleotide Sequences of Dog-Specific Primers for Quantitative Real-Time PCR

From: Differential expression of copper-associated and oxidative stress related proteins in a new variant of copper toxicosis in Doberman pinschers

Gene	Primer	Sequence (5'-3')	Tm (°C)	Product size (bp)	Accession number
GAPDH	Forward	TGT CCC CAC CCC CAA TGT ATC	58	100	AB038240
	Reversed	CTC CGA TGC CTG CTT CAC TAC CTT
HPRT	Forward	AGC TTG CTG GTG AAA AGG AC	56	100	L77488 /
	Reversed	TTA TAG TCA AGG GCA TAT CC			L77489
SOD1	Forward	TGG TGG TCC ACG AGA AAC GAG ATG	64	99	AF346417
	Reversed	CAA TGA CAC CAC AAG CCA AAC GAC T
CAT	Forward	TGA GCC CAG CCC TGA CAA AAT G	62	119	AB012918
	Reversed	CTC GAG CCC GGA AAG GAC AGT T
GSS	Forward	CTG GAG CGG CTG AAG GAC A	62	131	AY572226
	Reversed	AGC TCT GAG ATG CAC TGG ACA
GPX1	Forward	GCA ACC AGT TCG GGC ATC AG	62	123	AY572225
	Reversed	CGT TCA CCT CGC ACT TCT CAA AA
CCS	Forward	TGT GGC ATC ATC GCA CGC TCT G	64	96	AY572228
	Reversed	GGG CCG GCC TCG CTC CTC
p27KIP	Forward	CGG AGG GAC GCC AAA CAG G	60	90	AY455798
	Reversed	GTC CCG GGT CAA CTC TTC GTG
Bcl-2	Forward	TGG AGA GCG TCA ACC GGG AGA TGT	61	87	AB116145
	Reversed	AGG TGT GCA GAT GCC GGT TCA GGT
ATOX1	Forward	ACG CGG TCA GTC GGG TGC TC	67	137	AF179715
	Reversed	AAC GGC CTT TCC TGT TTT CTC CAG
COX17	Forward	ATC ATT GAG AAA GGA GAG GAG CAC	60	127	AY603041
	Reversed	TTC ATT CTT CAA GGA TTA TTC ATT TAC A
ATP7A	Forward	CTA CTG TCT GAT AAA CGG TCC CTA AA	50	99	AY603040
	Reversed	TGT GGT GTC ATC ATC TTC CCT GTA
ATP7B	Forward	GGT GGC CAT CGA CGG TGT GC	56	136	AY603039
	Reversed	CGT CTT GCG GTT GTC TCC TGT GAT
CP	Forward	AAT TCT CCC TTC TGT TTT TGG TT	62	97	AY572227
	Reversed	TTG TTT ACT TTC TCA GGG TGG TTA
MT1A	Forward	AGC TGC TGT GCC TGA TGT G	64	130	D84397
	Reversed	TAT ACA AAC GGG AAT GTA GAA AAC
MURR1	Forward	GAC CAA GCT GCT GTC ATT TCC AA	58	122	AY047597
	Reversed	TTG CCG TCA ACT CTC CAA CTC A
XIAP	Forward	ACT ATG TAT CAC TTG AGG CTC TGG TTT C	54	80	AY603038
	Reversed	AGT CTG GCT TGA TTC ATC TTG TGT ATG

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Comparative Hepatology