Transmission electron micrographs of liver periportal areas. (a), control, (b-d), after partial hepatectomy; (a) illustrates an intact histological relationship between liver sinusoidal endothelium (Ec) and neighboring liver parenchymal cells (Pc). Note the patent lumen (L). Inset depicts the intact cytoplasmic processes of endothelial cells bearing fenestrae (arrow). (b) Ten minutes after PHx, the surface area of the sinusoidal lumen (L) decreases and severe damage of the endothelial lining in the form of gaps is noted (arrow). Blood platelets (arrowhead) adhere to the damaged sinusoidal lumen. Inset shows a detailed image of blood platelets. (c) 24 hrs after PHx. Fat droplets (arrow) are evident in the cytoplasm of parenchymal cells. Gaps are still present (arrowhead). (d) 72 hrs after PHx reveals endothelial damage (arrowhead) and large fat droplets (arrow) within the parenchymal cells (compare with Figure 3c for the difference). Scale bars: 2 μm; Insets: 0.5 μm.