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Figure 3 | Comparative Hepatology

Figure 3

From: The aryl hydrocarbon receptor-mediated disruption of vitellogenin synthesis in the fish liver: Cross-talk between AHR- and ERα-signalling pathways

Figure 3

A) The negative effects of TCDD on the highly-induced vitellogenin gene expression. The salmon hepatocytes were treated with 100 nM E2 or E2 and increasing concentrations of TCDD (1–10 nM). Following a 12-h treatment period, total cellular RNA was isolated and the expression of the VTG gene was assessed by Northern blot analysis as described (Figure 1). The upper panel shows the expression of the VTG gene while the lower panel displays the ethidium bromide staining of the gel to demonstrate equal loading of the samples. The arrows indicate the position of 28S and 18S ribosomal RNAs. B) Effects of α-NF on the antiestrogenic activity of β-NF Slot blot hybridization analysis of VTG gene expression in cultured hepatocytes after treatment for 12 hrs with solvent (Control), fixed concentration of 17 β-estradiol (E2) (100 nM), E2 plus α-naphthoflavone (α-NF) (1 μM) or E2 plus increasing concentrations of α-naphthoflavone (from 1 to 103 nM). Total RNA (5 μg) was applied per slot and hybridized with a [α-32P]dCTP labelled VTG cDNA probe. Radioactivity in each slot was quantified using phosphoimager as photo stimulated luminiscence (PSL). The PSL values of three independent experiments and their means are shown in the plot diagram. Asterisk indicates significant difference (P < 0.05) with respect to the E2-treated sample (Dunnett's test).

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