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Figure 2 | Comparative Hepatology

Figure 2

From: The aryl hydrocarbon receptor-mediated disruption of vitellogenin synthesis in the fish liver: Cross-talk between AHR- and ERα-signalling pathways

Figure 2

The effects of α-naphthoflavone on the E2-induced vitellogenin mRNA levels. The cultured fish hepatocytes were treated with 10 nM E2 or E2 plus increasing concentrations of α-naphthoflavone (α-NF) (0–1 μM) for12 hrs. Total cellular RNA was subsequently isolated and 7.5 μg per sample was analyzed by slot blot hybridization. The membrane was hybridized with a [α-32P]dCTP labelled cDNA probe specific for VTG. The upper panel shows a representative slot blot membrane. The lower panel shows quantified radioactivity using phosphoimager as photo stimulated luminiscence (PSL). The PSL values of three independent experiments and their means are shown in the plot diagram.

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Comparative Hepatology

ISSN: 1476-5926