Skip to main content

Table 1 Neutrophil extravasation, liver injury and chlorotyrosine adduct formation during murine endotoxemia.

From: Chlorotyrosine protein adducts are reliable biomarkers of neutrophil-induced cytotoxicity in vivo

 

Number of Extravasated Neutrophils (per 10 HPF)

Plasma ALT Activities (U/L)

Necrosis (%)

Chlorotyrosine Staining (Intensity)

Controls

0 – 0

64 – 16

0 – 0

0

Gal/ET 6 h

23 – 3

41 – 9

15 – 2*

0

Gal/ET 6 h + ZVAD

6 – 2

18 – 13

2 – 1#

0

Gal/ET 7 h

186 – 30*,$

1252 – 369*,$

45 – 2*,$

++++

Gal/ET 7 h + ZVAD

19 – 5#

10 – 3#

3 – 1#

+

  1. Animals were sacrificed 6 or 7 h after Gal/ET treatment. Some animals received 2 doses of the pancaspase inhibitor ZVAD (10 mg/kg) at 3 and 4.5 h. Alanine aminotransferase (ALT) activities were measured in plasma. Liver sections were immunostained with the anti-Gr1 and anti-chlorotyrosine antibodies for evaluation of neutrophil extravasation and chlorotyrosine adduct formation, respectively. Necrosis was estimated in sections stained with H&E. Data represent means – SE of n = 4 animals per group. *P < 0.05 (compared to controls) $P < 0.05 (compared to Gal/ET6 h) #P < 0.05 (compared to Gal/ET alone).