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Table 1 Neutrophil extravasation, liver injury and chlorotyrosine adduct formation during murine endotoxemia.

From: Chlorotyrosine protein adducts are reliable biomarkers of neutrophil-induced cytotoxicity in vivo

  Number of Extravasated Neutrophils (per 10 HPF) Plasma ALT Activities (U/L) Necrosis (%) Chlorotyrosine Staining (Intensity)
Controls 0 – 0 64 – 16 0 – 0 0
Gal/ET 6 h 23 – 3 41 – 9 15 – 2* 0
Gal/ET 6 h + ZVAD 6 – 2 18 – 13 2 – 1# 0
Gal/ET 7 h 186 – 30*,$ 1252 – 369*,$ 45 – 2*,$ ++++
Gal/ET 7 h + ZVAD 19 – 5# 10 – 3# 3 – 1# +
  1. Animals were sacrificed 6 or 7 h after Gal/ET treatment. Some animals received 2 doses of the pancaspase inhibitor ZVAD (10 mg/kg) at 3 and 4.5 h. Alanine aminotransferase (ALT) activities were measured in plasma. Liver sections were immunostained with the anti-Gr1 and anti-chlorotyrosine antibodies for evaluation of neutrophil extravasation and chlorotyrosine adduct formation, respectively. Necrosis was estimated in sections stained with H&E. Data represent means – SE of n = 4 animals per group. *P < 0.05 (compared to controls) $P < 0.05 (compared to Gal/ET6 h) #P < 0.05 (compared to Gal/ET alone).