Figure 5
Immunohistochemical analysis of liver sections for nitrotyrosine (NT) protein adducts 1 and 6 h after administration of 300 mg/kg acetaminophen (AAP). One group of animals was pretreated with 10 mg/kg gadolinium chloride (A), the other group was pretreated with 100 mg/kg allopurinol (B). 1 h AAP-GdCl 3 (A): The liver was histologically normal but showed extensive staining in sinusoidal endothelial cells. 1 h AAP-Allopurinol (B): The liver was histologically normal with very limited NT staining in sinusoidal endothelial cells. 6 h AAP-GdCl 3 (C): Liver sections showed centrilobular necrosis and confluent hepatocellular staining for NT in centrilobular areas. 6 h AAP-Allopurinol (D): The liver was histologically normal with no evidence of NT staining.