Figure 2
From: Expression of the Peroxisome Proliferator-Activated Receptors (PPARs) in the Hepatic Stellate Cells
Increase of vitamin A-containing lipid droplets by addition of PPAR-gamma agonist in cultured HSCs. HSCs were cultured for 48 h in the presence of (A and D) 0.2% ethanol used as vehicle, or (B and E) 10 micromolar retinyl acetate, or (C and F) both 10 micromolar retinyl acetate and 10 micromolar PPAR-gamma agonist, ciglitazone. After fixation of the cells (phase contrast images: A, B, and C), autofluorescence emanated from vitamin A in the cytoplasmic lipid droplets was detected and recorded by using a fluorescence microscope equipped with a chilled CCD camera (D, E, and F).