Figure 1
From: Expression of the Peroxisome Proliferator-Activated Receptors (PPARs) in the Hepatic Stellate Cells
RT-PCR analysis for expression of PPAR subtypes (A) and PPAR-gamma isoform (B) in HSCs. Total RNA was isolated from HSCs (lanes 1-4 in Fig. 1-A and lanes 1-3 in Fig. 1-B) or 3T3-L1 adipocytes (lanes 4-6 in Fig. 1-B). (A): Total RNA was reverse transcribed, and then the cDNAs were amplified by PCR using a specific primer pair for PPAR-alpha (lane 1), PPAR-delta (lane 2), and PPAR-gamma (lane 3), as well as a primer pair for G3PDH as an internal control (lane 4). (B): Expression of mRNAs for PPAR-gamma isoform in HSCs was analyzed with the common primer pair for both PPAR gamma 1 and gamma 2 isoforms (lanes 1 and 4) and the specific primer pair for PPAR-gamma 2 isoform (lanes 2 and 5). Amplification of G3PDH cDNA was used as an internal control (lanes 3 and 6). A single band with the predicted size, 200 bp for each of PPAR-gamma isoforms and 460 bp for G3PDH was detected. M: DNA size markers.