Figure 1From: Expression of leptin receptors in hepatic sinusoidal cellsExpression of Ob-R mRNA in sinusoidal cells.Hepatic stellate cells (HSCs), sinusoidal endothelial cells (SECs), and Kupffer cells were isolated from Wistar rats, and cultured for 7 days, 3 days and 1 day, respectively. Total RNA (1 microgram) from each cell type was analyzed by RT-PCR for Ob-Ra, Ob-Rb, and beta-actin as described in Methods.Back to article page