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Table 6 Evaluation of apoptosis versus necrosis in HepG2 cultures incubated with 10 mM Pb(NO3)2, Pb(C2H3O3)2 or KNO3 and with conditioned medium from Kupffer cells incubated with10 mM Pb(NO3)2 for 24 hours.*

From: Kupffer cells promote lead nitrate-induced hepatocyte apoptosis via oxidative stress

Treatments 6 hours 8 hours 16 hours 24 hours 48 hours
  Necrosis Apoptosis Necrosis Apoptosis Necrosis Apoptosis Necrosis Apoptosis Necrosis Apoptosis
Control 2.00 0.80 2.05 0.75 2.50 1.20 2.70 3.51 3.06 4.01
Pb(NO3)2 1.80 0.90 2.05 1.17 2.84 1.15 13.73^ 4.35 25.00^ 9.20
Pb(C2H3O2)2 4.50^ 2.10 18.90^ 1.80 35.30^ 3.10 81.03^ 7.50^ 92.60^ 15.20^
KNO3 1.42 0.18 1.20 0.53 2.69 0.70 7.71 1.19 15.30^ 12.58^
C.M. 1 0.80 1.10 2.10 1.50 2.50 2.00 3.80 3.20 4.10 3.80
C.M. 2 1.70 3.10^ 3.20 4.50^ 2.80 10.10^ 3.20 18.50^ 3.80 25.30^
C.M. 3 0.90 2.00 1.80 3.10 1.90 2.30 2.50 7.10 2.90 4.20
  1. * Control animals were injected with saline. Apoptosis and necrosis were detected on slides of haematoxylin-eosin stained cells counting at least 150 cells in at least 10 randomly selected fields. C.M. 1 = conditioned medium collected from normal untreated Kupffer cell cultures. C.M. 2 = conditioned medium collected from Kupffer cell cultures incubated for 24 hours with Pb(NO3)2 (10 mM). C.M. 3 = conditioned medium collected from Kupffer cell cultures incubated for 24 h with KNO3 (10 mM). Data are given as percentages. Standard deviations did not exceed 5%. ^Significantly different in relation to Control (p < 0.05).