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Figure 7 | Comparative Hepatology

Figure 7

From: Eggshell and egg yolk proteins in fish: hepatic proteins for the next generation: oogenetic, population, and evolutionary implications of endocrine disruption

Figure 7

Immunohistochemical localization of vitellogenin (Vtg) in liver sections of control (a), nonylphenol- (b) and estradiol-17β-treated (c) juvenile Atlantic salmon (Salmon salar). Cellular Vtg levels were detected with mouse monoclonal antibody (BN-5) against salmon Vtg. Yellow colors show strong Vtg-specific staining and as demonstrated primarily in endothelial cells, hepatic sinusoids, and cytoplasm of hepatocytes (labeled C). Blue stains show nuclei of hepatocytes. Goat anti-mouse horseradish peroxidase (GAM-HRP) was used as secondary antibody. Reproduced from Arukwe et al. [143] with permission from Taylor and Francis http://www.tandf.co.uk.

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