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Figure 5 | Comparative Hepatology

Figure 5

From: Eggshell and egg yolk proteins in fish: hepatic proteins for the next generation: oogenetic, population, and evolutionary implications of endocrine disruption

Figure 5

Cross-reactivity of a monoclonal zebrafish (Danio rerio) vitellogenin antibody to different cyprinid fish species. Monoclonal mouse anti-zebrafish vitellogenin IgG JE-10D4 (Biosense Laboratories AS, Bergen, Norway) was used to probe a Western blot with samples of: (1) Pre-stained molecular weight standard (Bio-Rad), (2) purified zebrafish Vtg, (3) whole-body homogenate sample of estradiol-17β (E2) treated zebrafish, (4) whole-body homogenate sample of control zebrafish, (5) plasma sample of E2 treated carp (Cyprinus carpio), (6) plasma sample of control carp, (7) plasma sample of E2 treated fathead minnow (Pimephales promelas), (8) plasma sample of control fathead minnow, (9) plasma sample of E2 treated roach (Rutilus rutilus), (10) plasma sample of control roach. Reproduced with permission from Biosense Laboratories AS.

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Comparative Hepatology

ISSN: 1476-5926