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Table 3 Identification of cells after cultivation of elutriation fractions.

From: Liver sinusoidal endothelial cells represents an important blood clearance system in pigs

Fraction

FITC – FSA

Desmin

KC

Hepatocytes

1

32.1 (12.6)

63.9 (15.4)

4.0 (6.9)

0.0 (0.0)

2

80.4 (6.4)

10.9 (9.2)

7.0 (1.4)

1.7 (1.6)

3

66.2 (11.1)

7.1 (3.8)

15.2 (13.5)

11.5 (17.5)

4

61.0 (17.5)

2.1 (1.7)

10.9 (10.9)

26.0 (19.4)

  1. Prior to isolation of cells, pigs received FITC-labeled formaldehyde-treated serum albumin (FSA) intravenously. Stellate cells stained with monoclonal mouse anti-human desmin antibody and Kupffer cells (KC) stained with anti-pig macrophage antibodies. Hepatocytes were identified by simple morphology. Values are percent of total number of cells per culture (n = 3). The values are expressed as: mean (standard deviation).
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Comparative Hepatology

ISSN: 1476-5926